Supporting data for “Comparative Genomics in the genus Caenorhabditis and the Discovery of a Novel Toxin-Antidote System in C. elegans”

Abstract

This dataset accompanies the thesis “Comparative Genomics in the genus Caenorhabditis and the Discovery of a Novel Toxin-Antidote System in C. elegans.” It contains 37 files documenting the comparative genomic analyses and experimental work presented in the thesis. Main and supplementary figures cover genetic mapping, inheritance tests, RNA interference, reporter expression, microscopy, sequence comparisons, regional synteny, phylogenetic relationships, and the characterization of the sept-1/zina-1 toxin-antidote system. Additional plots summarize chromosome assignment quality, chromosome conservation, repeat composition, chromosome component sizes, and the presence or absence of developmental regulatory genes across Caenorhabditis species. Three true-scale locus plots provide synteny-based evidence for gene absence in selected species. Five Excel workbooks contain the underlying supporting information, including candidate gene annotations and alignment statistics, strain names and genotypes, software versions and links, cloning and genotyping primers, DNA constructs, expression cassettes, and reference and mutant allele sequences. The collection also includes editable and raster versions of a chromosome V mapping figure, along with embryo and fluorescence microscopy images. Together, these files provide the figures, supplementary tables, molecular resources, and comparative evidence needed to interpret the thesis results and trace the analyses from genomic patterns to experimental validation. They support future reuse of the associated scientific evidence.