gene editing

Analysis of SpCas9 on- and off-target effects in high efficiency multiplex editing in Arabidopsis

RNA-guided nucleases (RGNs), such as Cas9 from Streptococcus pyogenes (SpCas9), are widely used for plant genome editing. Previous surveys for off-targeting, the modification of unintended targets with similarity to the intended target, indicate high specificity of SpCas9 in plant cells. However, off-targeting has not...

Grass Carp Autophagy-Related Gene 5 Shows Inflammatory Regulation Function During Grass Carp Reovirus Infection

Grass carp (Ctenopharyngodon idella) aquaculture faces significant challenges due to grass carp hemorrhagic disease, which is instigated by the grass carp reovirus (GCRV). A major contributor to the high mortality rates associated with this disease is an excessive inflammatory response. Autophagy contributes to host di...

Doc2LoRA idea genes: reproduction code and archived intermediates for "Doc2LoRA Provides Decodable Representations of Scientific Ideas"

The reproduction workflow and the archived intermediate results for the paper. Everything needed to rebuild the manuscript's tables is here: the code is a snapshot of the trimmed reproduction repository, and the bundles hold the computed intermediates, so a reader does not have to re-derive them. Reproducing from the r...

PS1-1. Introducing Gene-Editing Semen for the Slick Mutation to Mitigate Fescue Toxicosis in Angus Cattle.

Tall fescue pastures support 40% of U.S. cow-calf herds, but fescue toxicosis causes heat stress and reduces animal performance and welfare. A mutation in the prolactin receptor (Slick mutation) gives cattle a sleek hair and reduces body temperature. This mutation does not occur naturally in Angus cattle, but can be...

Study of the genus Rosehip (Rosa) using molecular biological methods

This study presents a molecular genetic analysis of a rosehip sample using DNA barcoding and phylogenetic approaches. The aim of the research was species identification based on comparative analysis of nucleotide sequences of nuclear and chloroplast marker genes ITS, matK, rbcL, and trn. Genomic DNA was extracted from...

Targeted Mutations in d-ldh and budA Differentially Affect Adaptive Traits and Fermented Milk Quality in Companilactobacillus crustorum MN047

This study examined how targeted mutations in two pyruvate-associated genes, d-ldh and budA, affected adaptive phenotypes and fermented milk quality in Companilactobacillus crustorum MN047. Premature stop codons were introduced into d-ldh and budA by CRISPR-assisted base editing and verified by Sanger sequencing. The r...

Pathophysiology and Comprehensive Pharmacological Management of ATTR Cardiac Amyloidosis: Knowledge Gaps, Ongoing Clinical Trials, and Future Research Directions

The purpose of this review article is to discuss the current treatment modalities and ongoing clinical trials regarding cardiac amyloidosis (CA), specifically transthyretin cardiac amyloidosis (ATTR-CA). Amyloidosis refers to the extracellular deposition of insoluble amyloid fibrillar proteins. When these proteins are...

PS10-4. Effect of Mutating Porcine CYP17A1 for Altering Pregnenolone Metabolism to Control Boar Taint.

Cytochrome P450 17A1 (CYP17A1) interacts with cytochrome b5A (CYB5A) to regulate the metabolism of pregnenolone into 17α-hydroxypregnenolone (17OHP) and dehydroepiandrosterone (DHEA), androgens associated with superior growth performance in boars, or 5α-androst-16-ene-3-one (androstenone), a 16-androstene (16A) stero...

Replication Data for: Rice leaf structural adjustment to sustain photosynthetic traits under drought and produce more grain: Evidence from field studies of the 3K indica panel

Understanding plant traits that contribute to maintenance of physiological activities under drought is crucial for sustainable rice production. Leaf morphological characters together with UAV-based HTP measurements of canopy temperature (CT) and NDVI, leaf water potential (ψLeaf), photosynthetic traits including A, gs...

Transcriptomic Reanalysis Reveals Complement, Circadian, and Toll-Like Receptor Signaling Remodeling in Lactiplantibacillus plantarum-Induced Innate Immune Memory in Human Monocytes

The commensal bacterium Lactiplantibacillus plantarum is recognized for its ability to cause a state of innate immune memory in monocytes, characterized by the tolerogenic phenotype and lowered pro-inflammatory responses. This study provides an extensive re-examination of the transcriptomic dataset originating from hum...

101. Assessment of Published Bovine Guide RNA Design Practices.

CRISPR/Cas9 is the most widely applied gene-editing (GnEd) technology in livestock, with more than 200 published studies reporting the generation of live, gene-edited animals for agricultural applications. This system consists of a Cas9 nuclease that cleaves DNA and a 19-20 base pair guide RNA (gRNA) that directs the...

2.72P Optimization of intramuscular administration and in-vivo monitoring of gene-editing therapy in Duchenne muscular dystrophy