gene editing

CRISPR/CAS9-based gene editing in cancer therapy

Single-allele nanoscale mapping of regulatory variants.

Millions of genetic variants are linked to human disease but identifying underlying mechanisms is challenging because most variants are noncausal and lie within the noncoding genome. We developed a Micro Capture-C variant-to-function platform (MCCv) based on analysis of single-allele chromatin structure. This can ident...

Pioneering the next generation therapies in Parkinson's disease

Gene dosage effect and reduced core fucosylation in B lymphocytes of FUT8 heterozygous mice

Core fucosylation (alpha1,6-fucosylation) of N-glycans, catalyzed solely by fucosyltransferase 8 (FUT8), plays important roles in development, immunity, and antibody effector functions. Here we report the generation of Fut8 floxed mice using a CRISPR/Cas9-based Extreme Genome Editing (EGE) system, in which loxP sites f...

A naturally evolved mutation in cavefish mc3r reshapes energy homeostasis across development in a nutrient-limited environment

Food availability in natural habitats is often unpredictable and drives the selection of adaptive strategies in organisms to survive food scarcity. However, the genetic and molecular mechanisms that enable animals to survive under such nutritional extremes are poorly understood. Naturally adapted organisms therefore pr...

Introduction: Metals in Health and Environment

Metals occupy a unique and often paradoxical position at the interface between human health and the environment. Essential metals such as iron, zinc, copper, magnesium, and molybdenum are indispensable for numerous biological processes, yet their deficiency or excess can lead to serious health consequences. In contrast...

Biohacking Our Way to Health and Transhumanism

Biohacking emerged as a grassroots movement that seeks to democratize biology, biotechnology, and healthcare. Initially known as garage biology, biohacking developed from the convergence of synthetic biology, citizen and open-source science, and advances in biotechnology. What began as amateur experimentation in home l...

Maternal obesity imprints methylation marks in oocytes to drive intergenerational metabolic dysfunction.

Wheat Rust Diseases: Global Impact, Integrated Disease Management, and Emerging Biotechnological Innovations for Sustainable Crop Protection

Wheat (Triticum aestivum L.) remains one of the most important staple crops worldwide and serves as a primary source of calories and protein for billions of people. Global wheat production, however, continues to be threatened by rust diseases caused by Puccinia triticina, Puccinia striiformis f. sp. tritici and Puccini...

Programming primary human T cells to deliver gene editing machinery

Clinical applications of gene editors including CRISPR-Cas9 are limited by the cell-type specificity and tissue penetrance of current in vivo delivery options. Cell-mediated gene editor delivery could overcome these obstacles. To date, systems utilizing immortalized cell lines have demonstrated gene editor delivery in...

Current Evidence on Gene-Based and Epigenetic Therapeutic, Diagnostic, and Prognostic Strategies in Epithelial-Derived Carcinomas: A Scoping Review

Background: Epithelial-derived carcinomas exhibit genomic instability due to genetic mutation and epigenetic dysregulation. Mechanisms such as DNA methylation, histone modifications, and noncoding RNA regulation may lead to tumor progression, immune evasion, and treatment resistance. Presently, gene-based and epigeneti...

Complete assembly of Onobrychis viciifolia mitochondrial genome reveals evolutionary dynamics