An amplification‐free nanopore Cas9‐targeted sequencing (nCATS) platform is modified to achieve uniform coverage across 56 currently defined STR loci using a single test with genomic DNA from patient‐derived blood cells and to develop a dedicated analysis algorithm, STRiker, capable of identifying internal motif contex...
Seungbok Lee, Chan-Ju Jung, Minjeong Kim et al.· Advancement of science· 0 citations
DNA repair outcomes at nicks and mismatches introduced by base editors at the PCSK9 and HBG loci in human embryos are evaluated, finding that unlike Cas9-induced DNA breaks, base editor-induced lesions are efficiently repaired.
Stepan Jerabek, Chan-Ju Jung, M. Kappy et al.· Nature· 1 citation
A programmable gene replacement tool, named prime assembly (PA), which adapts prime editors to produce one or two pairs of 3'-flaps on both the genome and donor DNA, allowing megabase-scale genomic excision and/or kilobase-scale donor insertion at the gene of interest.
Hojun Jung, Bada Jeong, Yong-Woo Kim et al.· Nature Biotechnology· 1 citation· ⚡1
A single-nucleotide resolution ABE created by fusing a nickase Cas9, nCas9-H840A, with the deaminase domain of ADAR, which acts on DNA:RNA hybrids, instead of TadA, which acts on single-stranded DNA in conventional ABEs.
Hyeon Woo Im, Bada Jeong, Yeji Lee et al.· Nature Biotechnology· 1 citation
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
