Production of Lentiviral Vectors Encoding the CRISPR-Cas13d System for RNA Targeting.
A detailed protocol for the production of high-titer, third-generation LVs with a dual-expression cassette for Cas13d and a customizable guide RNA is described, which can be used for stable transduction and efficient RNA knockdown in a broad range of mammalian cell types.
R. Stilhano, L. Martin
· Methods in molecular biology · 0 citations