Author

Jure Bohinc

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#gene editing Open access Sep 2026

Decoupling reactogenicity from efficacy enables safer mRNA vaccines, AAV gene delivery, and in vivo base editing.

A unifying innate-sensing architecture for nucleic-acid therapeutics is defined and it is established that reactogenicity is not required for effective vaccination or gene delivery, enabling a broadly applicable strategy to widen the safety window of mRNA medicines, viral vectors, and in vivo genome editing.

D. Lainšček, Š. Malenšek, V. Forstnerič et al. · Molecular Therapy · 0 citations

#protein folding Open access Sep 2026

Production and Purification of Cas9 Protein for Ribonucleoprotein Complex Formation.

A short procedure for assembling Cas9 RNPs with in vitro-transcribed guide RNAs is provided, making the protocol directly applicable to CRISPR RNP delivery in mammalian cells.

M. Skrbinek, Jure Bohinc · Methods in molecular biology · 0 citations

Review Open access Jul 2026

In vivo delivery strategies for therapeutic CRISPR genome editing

A comprehensive review of delivery modalities of CRISPR systems solely in vivo that underpin their therapeutic translation and outlines the remaining barriers to durable, tissue-selective, and broadly deployable CRISPR therapeutics is provided.

L. Martin, Jure Bohinc, Alessandra Recchia et al. · International Journal on Bio... · 0 citations