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#gene editing Open access Aug 2026

Efficient in-vitro regeneration and transformation for CRISPR/Cas9-mediated genome editing of phytoene desaturase (PDS) gene in pea (Pisum sativum L.)

The present study addresses optimization of in-vitro regeneration via direct organogenesis and Agrobacterium-mediated genetic transformation, enabling efficient multiplex CRISPR/Cas9-based genome editing of the phytoene desaturase (PsPDS) gene in pea. Pea (Pisum sativum L.) is an important legume crop valued for food,...

Hardeep Singh, Pankaj Kumar, Vishal Sharma et al. · 0 citations
Open access Jul 2026

CRISPR/Cas9-based gene-editing platform development by targeting genes encoding magnesium chelatase and phytoene desaturase in sugarbeet (Beta vulgaris L.)

The results demonstrate the successful deployment of CRISPR/Cas9 for targeted genome engineering in sugarbeet and establish a reliable platform for future gene-editing efforts aimed at enhancing resistance to a wide range of pathogens and diseases affecting the crop.

Z. Khan, Tinley Hathaway, C. Chu et al. · 0 citations
Open access Jul 2026

AgroGem: A Rapid and Scalable Transient Transformation System for Functional Genetics in Multiple Plant Species

AgroGem is developed, an efficient Agrobacterium-mediated transient transformation system utilizing a geminiviral replicon-based T-DNA vector for Arabidopsis and Brassicaceae species and established as a robust, scalable, and versatile platform for genome editing, DNA repair analysis, and functional genetics in plants.

Sheng-Song Guo, Oliver Schlegel, Jitesh Kumar et al. · 0 citations
Aug 2026

SAM-Targeted CRISPR-Cas9 RNP Delivery Combined with Leaf Regeneration Enables DNA-Free, Non-Chimeric Genome Editing in ‘Fuji’ Apple

iPB-REG is established as a practical strategy for producing uniform genome-edited fruit trees and provide a valuable platform for DNA-free genetic improvement and functional genomics in clonally propagated perennial crops.

C. Nishitani, Nozomi Tsujino, Misa Kuroki et al. · 0 citations
Aug 2026

A Novel, Simple, and Highly Efficient Dual-sgRNA CRISPR/Cas9-Based Editing System in the Filamentous Fungus Fusarium oxysporum

A novel highly efficient CRISPR/Cas9-based dual-sgRNA expression editing system for F. oxysporum suitable not only for single-gene editing but also for large-fragment deletion and multiplex gene editing, although the editing efficiency is somewhat lower.

Wangjie Zhu, Jiao Liao, Yuanyuan Liu et al. · 0 citations

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