Aug 2026· Transgenic research· Vol 35· 0 citations· 53 references
Medicine
TL;DR
The first isolation and characterization of the buffalo EF1α1 promoter is reported, demonstrating its strong gene expression activity both in vitro across diverse cultured cell types and in vivo across multiple mouse organs, establishing the bbEF1α1 promoter as a powerful ubiquitous driver of gene expression.
This work cloned the promoter of the housekeeping gene eukaryotic translation elongation factor 1α (EF1Α, AAEL017096) and confirmed its transcriptional activity, and truncated the U6 promoter, expanding the genetic toolkit for Ae.
Findings establish COMPACTs as a practical alternative to native promoters for various applications, including cell therapies, gene therapies, and biomanufacturing.
Chaja Katzman, S. Matusevich, Shir-Liya Dadon et al.· bioRxiv· 0 citations
Precise spatial restriction of transgene expression is essential for safe in vivo production of bioactive proteins; however, regulatory elements that confer tissue specificity often span large genomic regions and are incompatible with size-limited viral vectors. Here, we report a compact synthetic promoter-feedback cir...
Yoshinori Kawabe, Takayuki Otsubo, Reina Obata et al.· New Biotechnology· 0 citations
The CAG promoter is poorly suited for transgene expression in the lung and brain cells due to promoter silencing and is suboptimal for expression in the liver cells because of relatively low activity; these findings should be taken into account in the development of gene therapy products.
R. L. Anisimov, N. V. Nikiforova, E. S. Ivanov et al.· Biological Products. Prevent...· 0 citations
The findings suggest that transient trim25 knockdown may alleviate intracellular restriction of exogenous reporter expression in PAC2 and CST cells and may provide an affordable approach for improving molecular manipulation in selected fish cell systems.
This review systematically summarizes the features of Bacillus subtilis expression systems, the regulatory effects of various promoters on heterologous expression efficiency, and rational modification strategies of promoter core regions, offering valuable references for the rational development of high-efficiency promo...
Man-Fei Li, Meng Zheng, Peng-Yuan Wang et al.· Journal of Microbiological M...· 0 citations
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