TL;DR
A brief summary of the most important considerations for expression of heterologous genes, including expression strain selection, codon optimization, the choice of insertion loci, as well as potential protein fusions for targeting, localization, purification, and detection, are provided.
A streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeric fluorescent protein under the control of two promoters of choice is described, providing a fast and flexible cloning strategy for protein expression and subcellular localization analyses in mycobacteria.
Detailed instructions are provided for generating conditional gene deletions in the corn phytopathogenic fungus Ustilago maydis, while infecting corn plants through transcriptional control of the recombinase by fungal promoters that are specifically activated during the biotrophic phase of fungal growth.
At the Federal University of São Carlos (São Carlos, SP, Brazil), third-year biotechnology undergraduate students are required to take the theoretical and practical course "Expression and Purification of Recombinant Proteins”. The course includes laboratory exercises that show how to express proteins in the Pichia past...
This engineering method allows for endogenous expression of the Soc-mCherry recombinant protein within Escherichia coli cells during phage replication, after which the recombinant protein spontaneously assembles onto the capsid of the engineered phage.
High-yield recombinant protein expression is a critical requirement in biopharmaceutical development. The pET vector family is among the most widely used systems for protein production in Escherichia coli. Shilling et al. (2020) reported that insertion of a GAGA sequence within the T7lac promoter region of pET28a enhan...
This research provides a foundation for future isolation and characterisation studies to discover novel bioactive natural products through two activation strategies, namely heterologous expression and transcriptional activation in the native host via promoter engineering and regulatory gene overexpression.
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