Although genetically encoded fluorescent sensors are fundamental tools to gain an understanding of intracellular nitric oxide (NO) dynamics, their development is still limited. We recently introduced the blue-emitting fluorescent protein (FP) mTagBFP2 as a NO sensor, where the chromophoric group formed upon S-nitrosy...
Lucia Bellanova, Antonio Scarano, Carlotta Viappiani et al.· Chemical & Biomedical
Im...· 0 citations
Natural compounds are promising molecules with pharmaceutical, environmental and biotechnological applications. The catecholate siderophore myxochelin A displays promising pharmaceutical characteristics, inhibiting human 5-lipoxygenase thereby impacting inflammatory reactions and cancer development. It is naturally pro...
Franz Kofler, Astrid Wirtz, Amelie Jäger et al.· New Biotechnology· 0 citations
A brief summary of the most important considerations for expression of heterologous genes, including expression strain selection, codon optimization, the choice of insertion loci, as well as potential protein fusions for targeting, localization, purification, and detection, are provided.
S. Wegmann, Lesley Plücker, T. Drepper et al.· Methods in molecular biology· 0 citations
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