High-speed atomic force microscopy (HS-AFM) experiments provide unique insights into nanoscale biological processes by directly visualizing the surface topography dynamics of biomolecules, such as proteins, over extended periods of time and generating large imaging data sets. However, the lack of computational methods...
R. Amyot, Soma Yamamoto, Hiroki Konno et al.· bioRxiv· 0 citations
Electron transfer (ET) is involved in many fundamental biological processes. While spectroscopic and electrochemical techniques have established the theoretical framework governing protein ET, they typically capture average responses across large populations or characterize ET in fixed molecular arrangements. Electroch...
J. Samitier, A. Lagunas· Frontiers in Biophysics· 0 citations
High-speed atomic force microscopy (HS-AFM) enables visualization of biomolecular dynamics in solution with high spatiotemporal resolution but requires substrates that support stable adsorption without perturbing their structure or dynamics. Mica is widely used as an HS-AFM substrate because it provides an atomically f...
Integrins are bidirectional mechanochemical receptors that transmit signals upon ligand binding to the cytoskeleton (outside-in) and cytoskeletal forces back across the membrane (inside-out) to the integrin-ligand bond. Integrins are activated prior to ligand binding, which involves large conformational rearrangements...
Mandana Christine Hadawi, Bin Li, Monja Fink et al.· Biophysical Journal· 0 citations
High hydrostatic pressure provides a unique and sensitive perturbation to protein structure, enabling direct measurement of volume changes associated with folding, misfolding, and assembly. Because pressure perturbs noncovalent forces while preserving covalent integrity, it reveals conformational intermediates that o...
J. L. Silva, G. C. de Andrade, M. A. Marques et al.· ACS Applied Polymer Material...· 0 citations
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