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Review Open access Sep 2026

High-Speed AFM of Membrane Proteins: From Dynamic Imaging to Integrative Structural Biology

High-speed atomic force microscopy (HS-AFM) enables direct, label-free visualization of membrane proteins in lipid bilayers with nanometer spatial resolution and millisecond temporal resolution. Recent studies revealed transient conformational states, dynamic oligomerization changes, and membrane-mediated organization...

Eunji Shin, Jonathan Mount, Simon Scheuring · 0 citations
Open access Sep 2026

BioAFMviewer AFM Analysis Arena A3 – an interactive software platform for automated analysis of HS-AFM imaging

High-speed atomic force microscopy (HS-AFM) experiments provide unique insights into nanoscale biological processes by directly visualizing the surface topography dynamics of biomolecules, such as proteins, over extended periods of time and generating large imaging data sets. However, the lack of computational methods...

R. Amyot, Soma Yamamoto, Hiroki Konno et al. · 0 citations
Review Open access Sep 2026

Electrochemical scanning tunneling microscopy as a tool for probing protein function: from electron transfer to molecular recognition and chemosensation

Electron transfer (ET) is involved in many fundamental biological processes. While spectroscopic and electrochemical techniques have established the theoretical framework governing protein ET, they typically capture average responses across large populations or characterize ET in fixed molecular arrangements. Electroch...

J. Samitier, A. Lagunas · 0 citations
Open access Sep 2026

Silane-Based Methyl- and Carboxyl-Functionalized Mica Substrates for High-Speed Atomic Force Microscopy

High-speed atomic force microscopy (HS-AFM) enables visualization of biomolecular dynamics in solution with high spatiotemporal resolution but requires substrates that support stable adsorption without perturbing their structure or dynamics. Mica is widely used as an HS-AFM substrate because it provides an atomically f...

Kaho Nakamoto, Yuna Goto, Sohta Yamamoto et al. · 0 citations
Aug 2026

Single-Molecule Force Spectroscopy Reveals a High-Force Requirement for Dissociation of Integrin Transmembrane Domains.

Integrins are bidirectional mechanochemical receptors that transmit signals upon ligand binding to the cytoskeleton (outside-in) and cytoskeletal forces back across the membrane (inside-out) to the integrin-ligand bond. Integrins are activated prior to ligand binding, which involves large conformational rearrangements...

Mandana Christine Hadawi, Bin Li, Monja Fink et al. · 0 citations
#protein folding Review Open access Oct 2026

Biomolecules under Pressure: Protein Phase Transitions from Folding to Misfolding, Aggregation, and Condensation

High hydrostatic pressure provides a unique and sensitive perturbation to protein structure, enabling direct measurement of volume changes associated with folding, misfolding, and assembly. Because pressure perturbs noncovalent forces while preserving covalent integrity, it reveals conformational intermediates that o...

J. L. Silva, G. C. de Andrade, M. A. Marques et al. · 0 citations

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