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Derivation of a new induced pluripotent stem cell line ORIONi004-A for modelling Duchenne muscular dystrophy.

Dominika Hajduchova Stanislava Suroviaková S. Mersakova V. Nosáľ D. Brany Martin Rehák Adela Blahova Jakub Chmelar Oľga Chodelková Katarina Leskova Majdova Henrieta Škovierová Veronika Kucháriková S. Nováková Z. Hatoková Bibiána Baďurová J. Marcinek M. Péč R. Petrovič M. Fisherova M. Juhosová B. Mitrušková M. Pokusa A. Evinova Marek Samec M. Samoš Andreas Nicodemou L. Danišovič D. Dobrota E. Kurča K. Kolejáková J. Chandoga L. Plank M. Péč R. Péčová E. Halašová M. Kalman J. Strnádel

Aug 2026 · Stem Cell Research · Vol 95, pp. 104077 · 0 citations · 4 references

Medicine

TL;DR

Peripheral blood was used as source for primary cells and subsequently reprogrammed into induced pluripotent stem cells with synthetic Sendai vector, which showed the expression of pluripotency factors and the ability to differentiate into all three germ layers.

Abstract

Anew in vitromodel for modeling Duchenne muscular dystrophy, derived from pediatric patient with a unique mutation in DMD gene is introduced. Peripheral blood was used as asource for primary cells (mononuclear cells) and subsequently reprogrammed into induced pluripotent stem cells with synthetic Sendai vector. Reprogrammed iPS cells showed the expression of pluripotency factors and the ability to differentiateinto all three germ layers.

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