Open access
Derivation of a new induced pluripotent stem cell line ORIONi004-A for modelling Duchenne muscular dystrophy.
Dominika Hajduchova
Stanislava Suroviaková
S. Mersakova
V. Nosáľ
D. Brany
M. Rehák
Adela Blahova
Jakub Chmelar
Oľga Chodelková
Katarina Leskova Majdova
Henrieta Škovierová
Veronika Kucháriková
S. Nováková
Z. Hatoková
Bibiána Baďurová
J. Marcinek
M. Péč
R. Petrovič
M. Fisherova
M. Juhosová
B. Mitrušková
M. Pokusa
A. Evinova
Marek Samec
M. Samoš
Andreas Nicodemou
L. Danišovič
Dusan Dobrota
E. Kurča
K. Kolejáková
J. Chandoga
L. Plank
M. Péč
R. Péčová
E. Halašová
M. Kalman
J. Strnádel
Medicine
Abstract
Anew in vitromodel for modeling Duchenne muscular dystrophy, derived from pediatric patient with a unique mutation in DMD gene is introduced. Peripheral blood was used as asource for primary cells (mononuclear cells) and subsequently reprogrammed into induced pluripotent stem cells with synthetic Sendai vector. Reprogrammed iPS cells showed the expression of pluripotency factors and the ability to differentiateinto all three germ layers.