Marker-Assisted Development of Rice Line G163 Combining Pi2-Mediated Blast Resistance, Xa7-Mediated Bacterial Blight Resistance, and Favorable Grain Quality
Rice blast and bacterial blight are two major diseases that threaten rice production worldwide. Developing rice cultivars with both disease resistance and desirable grain quality is an important objective in modern rice breeding. In this study, R1179 carrying Pi2, Wxb and Alkb was crossed with IRBB7 carrying Xa7, Wxb and Alkb. Marker-assisted selection (MAS) was applied to an F2 population of 1123 plants, and a line, G163, combining Pi2, Xa7, Wxb and Alkb, was developed. Disease resistance evaluation showed that G163 retained the blast resistance conferred by Pi2 and the bacterial blight resistance conferred by Xa7, exhibiting resistance to the tested isolates of both pathogens. Grain quality assessment indicated that G163 possessed favorable milling, appearance and eating quality traits. Rapid Visco Analyzer (RVA) profiling further indicated desirable starch pasting properties. In conclusion, the rice line G163 carrying Pi2, Xa7, Wxb and Alkb was successfully developed through MAS. The results provide a useful germplasm resource for breeding rice cultivars with combined disease resistance and desirable grain quality.
Background: Uma is a high-yielding, popular red-kernelled rice variety of Kerala; however, the occurrence of BB disease often causes severe yield reductions in this cultivar. Host plant resistance based on multiple genes is the most effective measure to impart durable resistance. Hence, efforts were undertaken to intro...
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Bacterial leaf blight (BLB) of rice, caused by Xanthomonas oryzae pv. oryzae, is a major constraint on rice production, and the use of resistant genotypes is an important approach to disease management. The present study screened 40 rice genotypes for BLB resistance using morphological evaluation and molecular markers...
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M. Sriram, K. Amudha, Swaminathan Manonmani et al.· Biological Research· 0 citations
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The present study aimed to develop blast-resistant homozygous recombinant lines through marker-assisted selection (MAS) in segregating populations derived from the cross between Pusa Samba 1850 and CO55. Unlike backcross-based approaches, selection was focused on F2 derived recombinants and their advancement to homozyg...
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