Characterisation of advanced breeding lines for bacterial leaf blight resistance by morpho-molecular screening in rice (Oryza sativa L.)
Abstract
Sixty advanced breeding lines of rice were evaluated for resistance to bacterial leaf blight (BLB) both phenotypically and genotypically at Regional Agricultural Research Station (RARS), Maruteru, Andhra Pradesh, India during kharif, 2023. Phenotypic screening was performed following the International Rice Research Institute (IRRI), standard evaluation system (SES) scale. Among the sixty entries, seven genotypes, 251-3-3-2, MS-1, NLR-163, NLR-164, NLR-945, CM-469 and BM-587, exhibited a highly resistant (HR) reaction with a score of 1, comparable to the resistant check RP Bio-226. Twenty-two lines expressed BLB severity of 13–25 % (score 5) and were categorised as moderately resistant (MR). Molecular profiling using gene-specific simple sequence repeats (SSR) markers revealed significant variation in resistance genedistribution. The presence of xa5, xa13, Xa21, Xa4 and Xa10 was detected in 9, 4, 23, 44 and 20 lines respectively. Two-gene combinations were identified in 20 genotypes, whereas 11 genotypes possessed three-gene combinations, highlighting the effectiveness of gene stacking for broad-spectrum BLB resistance. Notably, lines 251-3-3-2, MS-1, NLR-164, NLR-945 and BM-587 exhibited congruence between phenotypic resistance (score 1) and the presence of multiple resistance genes, indicating their potential as elite donors for BLB resistance introgression. Yield performance coupled with gene pyramiding further strengthened the value of several genotypes. The genotypes VP-R-157 (Xa4+xa13) recorded 22.3 g/plant, BM-588 (xa5+Xa21) yielded 21.2 g/plant, while CM-467 (Xa4+xa5+Xa10+Xa21) produced 25.1 g/plant. The genotypes CM-470, CM-446 and CM-449 yielded 26.3 g, 21.0 g and 21.5 g/plant respectively, each carrying the Xa4+xa5 gene combination. These genotypes hold substantial promise for direct varietal release or utilisation in hybridisation programs aimed at developing high-yielding, BLB-resistant rice cultivars.