TL;DR
This study overcomes a key bottleneck in CRISPR/Cas delivery to small insect embryos, opening new avenues for rapid, high-throughput, and cost-effective RNP delivery methods in insect embryos.
Transgene-free genome edited plants were regenerated from protoplasts, representing the first report of RNP mediated genome editing in eggplant protoplasts.
This work establishes a highly effective electroporation strategy, augmented by nanotechnology, for the delivery of mRNA and RNP complexes, enabling high-efficiency protein expression and gene editing in zebrafish embryos, with broad potential applications in aquatic biotechnology.
Cpf1-based genome editing tools were developed for N. punctiforme, and a single-step cloning strategy was devised, allowing for rapid assembly of editing plasmids, and improved conjugation protocols for genetic transfer from E. coli to N. punctiforme were implemented.
An in vitro CRISPR/Cas9–RNP system efficiently cleaves CcYUC1 in Coffea canephora, establishing a foundation for DNA-free genome editing in coffee and providing a basis for future functional studies of auxin biosynthesis during somatic embryogenesis.
A transgenic Lytechinus pictus line that constitutively and ubiquitously expresses a large fragment of mNeonGreen is used, providing a streamlined, scalable method for endogenous protein visualization in echinoderm embryos and a valuable resource for studying gene function, morphogenesis, and toxicant response in this...
The established CRISPR/Cas12a-based detection platform provides a robust technical tool for early warning and on-site rapid screening of DIV1, facilitating timely disease control and risk management in shrimp farming.
General Science
General Science
GPT-Lab
· gpt-lab.eu
Oct 8, 2026
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