Application to a human sample from a patient with type 2 Diabetes Mellitus recovered a dysbiotic signature consistent with the literature, including reduced Firmicutes abundance, elevated Bacteroidetes and Proteobacteria, and a predominance of clinical associations within metabolic and gastrointestinal categories.
Rodrigo Lima Andrade, Tayná da Silva Fiúza, J. Kroll et al.· bioRxiv· 0 citations
Taxonomic profiling is fundamental to microbiome research, yet achieving high species-level accuracy remains challenging for complex communities that span bacteria, viruses, eukaryotes, and archaea, and these limitations are exacerbated in low-biomass, host-dominated samples. We introduce Metax, a cross-domain taxonomi...
Zhi-Luo Deng, Nasim Safaei, A. McHardy· Cell· 0 citations
Background
Metagenomic next-generation sequencing (NGS) enables comprehensive detection of a broad spectrum of microorganisms. However, its clinical application faces two major challenges: the development of appropriate microbiome-based biomarkers and the need to ensure the reproducibility and quality of the microbiome...
Dokyun Kim, Songhee Lee, Da-Young Kang et al.· Annals of Laboratory Medicin...· 0 citations
The gut microbiome, a complex community of
microorganisms in the gastrointestinal tract, plays a
vital role in host health, nutrition and adaptability.
Advances in next-generation sequencing and
metagenomics have deepened our understanding of
how diet and environmental factors shape microbial
diversity. In wild animals...
Pritee Chunarkar, Manoj Malavika, Rahul Gupta et al.· Research journal of biotechn...· 0 citations
The recognition of the urinary microbiome (urobiome) has challenged the long-standing paradigm of urinary tract sterility, revealing diverse microbial communities associated with both urinary health and disease. However, the characterization of the urobiome remains highly variable across studies, particularly due to th...
Viviana Méndez-Sacta, Solmayra Agreda Orellana, Luz María Ayavaca-Tapia et al.· Frontiers in Microbiology· 0 citations
Intensive efforts have been made to capture structural features of the gut microbiome for assessing microbiota status in relation to host health. However, gut microbiome function emerges not only from individual microbial taxa but also from their organization into ecological guilds, complicating microbiome structure-fu...