This study constructed a pH-responsive P-TN/SF@Fe-Cur composite coating that demonstrated significant anti-infective, anti-inflammatory, antioxidant, pro-angiogenic, and pro-osteogenic effects in rat subcutaneous infection and femoral defect models.
Abstract
To address the multifaceted clinical challenges of titanium implants in infection, oxidative stress, inflammation, and osseointegration, there is an urgent need to develop multifunctional smart coatings capable of synergistically regulating the local microenvironment. This study constructed a pH-responsive P-TN/SF@Fe-Cur composite coating by loading antibacterial peptide Pt5-1c onto titanium dioxide nanotubes (TN) and utilizing methacrylated silk protein-based hydrogel (SFMA) loaded with iron-curcumin nanoparticles (Fe-Cur NPs) as the sealing layer. Within acidic infectious microenvironments, this coating intelligently released Pt5-1c, Fe3+, and curcumin: Pt5-1c exerted broad-spectrum antibacterial activity by disrupting bacterial cell membranes, resulting in a 77% reduction in viable bacterial counts; Fe3+ might contribute to stabilizing with Pt5-1c to promote endothelial cell migration and expression of angiogenesis markers (CD31, VEGF); while Fe-Cur and Pt5-1c jointly induced M2 polarization of macrophages and effectively scavenged reactive oxygen species, with an 86.75% DPPH radical scavenging rate. Furthermore, this coating exhibited dual functions of direct osteogenesis and immune-regulated osteogenesis, synergistically promoting osteoblastic differentiation with core osteogenic genes (ALP, OCN, COL1a1, RUNX2) upregulated by more than 2.5-fold through multiple pathways including activation of innate immune response regulation, sodium ion transmembrane transporter activity, and glutathione metabolism. In rat subcutaneous infection and femoral defect models, this coating demonstrated significant anti-infective, anti-inflammatory, antioxidant, pro-angiogenic, and pro-osteogenic effects. This study has proposed a novel strategy for fabricating functionally integrated titanium implant coatings with the capacity for synergistic microenvironmental modulation.
This work demonstrates a safe and effective strategy for combating MDR infections through the combined action of photothermal therapy and nanozyme catalysis, offering promising potential for clinical wound management.
Weiwei Zhang, Lixiang Fan, Xuanjun Zhang et al.· ACS Applied Materials and In...· 0 citations
A multifunctional hydrogel patch developed by chemically modifying chitosan with N-acetylsulfonyl chloride and forming a cross-linked network with polyvinylpyrrolidone (PVP) represents a promising multifunctional dressing for the effective management of infected wounds.
Insha Kakroo, Nayeema Gull, Insha Mehraj et al.· ACS Applied Bio Materials· 0 citations
The skin serves as the largest protective barrier organ of the human body and is easily impaired by trauma, infection and chronic diseases. Efficient wound dressings are indispensable for repairing infected wounds. Isochlorogenic acid A (IAA), the core active ingredient of Shanyinhua, has superior anti-inflammatory and antibacterial effects. However, low water solubility and weak structural stability restrict its direct application in wound treatment. In this work, IAA@Fe(III) nanoparticles (IAA@Fe(III) NPs) were synthesized through self-assembly and loaded into cross-linked amylopectin (Amy)/carboxymethyl chitosan (CMCS) (AC hydrogel) to construct Amy/CMCS@NPs composite dressings. Characterizations demonstrated that nanoparticles displayed a uniform spherical shape with a size of 114.20 ± 2.29 nm and stable coordination. The hydrogel featured a dense porous structure and outstanding mechanical performance, self-healing ability, adhesion, and swelling properties. In vitro tests proved that 50 mg/mL composite hydrogel exerted nearly 100% bacteriostatic activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with good biocompatibility, and enhanced cell migration capacity. In vivo assays indicated an 86.5% wound healing rate at day 7. This dressing could downregulate Tumor Necrosis Factor-α (TNF-α) and Interleukin-1β (IL-1β), upregulate Cluster of Differentiation 31 (CD31) and Vascular Endothelial Growth Factor (VEGF), and accelerate wound repair. This study provides a theoretical and experimental basis for the exploitation of IAA-based wound dressings and high-value utilization of Shanyinhua resources.
Hui Li, Danli Peng, Zhijia Wang et al.· Gels· 0 citations
The reduction in microbial adhesion, coupled with enhanced osteoblast attachment, suggests a synergistic effect that may increase the success rate of implant integration at an early stage.
Bartosz Mielan, M. Pajączkowska, Joanna Nowicka et al.· International Journal of Mol...· 0 citations
A core–shell-type ZnO@ZIF-8/Ag nanocomposite material that combines high antibacterial activity with excellent biocompatibility offers a strategy for developing therapies against drug-resistant bacterial infections with the potential for clinical application.
Infected burn wounds are characterized by bacterial invasion, oxidative stress, and persistent inflammation, which severely impair tissue regeneration. Herein, we report a crosslinker-free, hydrogen-bonded cationic guar gum hydrogel (CBBM) co-loaded with berberine (BBR) and MnO2-coated black phosphorus nanosheets (BPNS@MnO2) for the microenvironment-adaptive treatment of infected burn wounds. The dynamic guar gum network endowed the hydrogel with injectability, self-healing ability, and conformal adaptability. BPNS@MnO2 exhibited pH-dependent enzyme-like activities, including OXD-like antibacterial activity under acidic conditions and SOD-/CAT-like ROS-scavenging activity under near-neutral conditions and endowed the hydrogel with NIR-triggered photothermal responsiveness. Moreover, NIR irradiation further enhanced the antibacterial efficacy and moderately enhanced BBR release from the hydrogel. In vitro, CBBM combined with NIR irradiation achieved potent antibacterial efficacy against S. aureus and E. coli under the tested conditions and reduced intracellular ROS levels. In vivo, the CBBM + NIR treatment accelerated infected burn wound healing, achieving a wound closure rate of 97.53 ± 2.01% by day 14, accompanied by reduced inflammation, enhanced collagen deposition, and increased expression of the angiogenesis-related markers VEGF and CD31. These results demonstrate that the CBBM hydrogel is a promising multifunctional guar gum-based dressing for infected burn wound healing by integrating local drug delivery, catalytic regulation, and photothermal activation.
Ziyi Zhao, Yanxiang Sang, Benyan Zheng et al.· International Journal of Bio...· 0 citations