The structural, evolutionary, and expression characteristics of the BjNDPK family are described and candidate genes for future functional studies of abiotic-stress responses in polyploid B. juncea are provided.
Functional characterization demonstrated that transgenic tobacco plants overexpressing AsGRF17 displayed significantly enhanced drought tolerance compared with wild-type plants, and this study provides new insights into the molecular mechanisms underlying AsGRF17 -mediated drought tolerance in oat.
Jingyan Li, Shi Tong, Kailian Li et al.· 0 citations
A systematic analysis of the MtPLATZ gene family in M. truncatula is provided, offering a valuable reference for functional studies and genetic improvement of stress tolerance in legumes.
Meprin and TRAF-C homology (MATH) proteins function as adaptor molecules and components of E3 ubiquitin ligase complexes. They link receptor-like kinase signalling to downstream regulatory pathways in plants. MATH proteins coordinate growth, hormone signalling, and responses to abiotic stress by modulating ubiquitin-dependent proteostasis. Despite their importance, however, little is known about the diversity, function, and specific regulatory role of the
MATH
gene family in
Brassica napus
.
We identified 151
BnMATH
family genes distributed across the 19 chromosomes of
B. napus
using a hidden Markov model-based genome-wide search followed by domain validation. Comparative phylogenetic and structural analyses classified these genes into four conserved clades, revealing that extensive segmental and tandem duplication events had driven the family expansion. Promoter analysis revealed more than 6,000 cis-acting regulatory elements associated with hormone- and stress-responsive gene expression. A total of 44 miRNA families targeting BnMATH genes were identified, among which 10 have been previously validated to be involved in biological processes. Transcriptome profiling combined with qRT-PCR validation revealed pronounced tissue-specific and abiotic-stress-responsive expression patterns. Notably,
BnMATH06
,
BnMATH92,
and
BnMATH135
were strongly induced by salt and drought stress, suggesting a potential role in stress adaptation.
These findings deepen our understanding of the
MATH
gene family and provide a robust foundation for future functional genomics research targeting their specific biological roles, particularly in hormone-driven regulation and adaptation to abiotic stress.
Fatima Maliha, Wenyu Wu, Li Long et al.· BMC Plant Biology· 0 citations
This study systematically characterized the Aux/IAA gene family in pumpkin, highlighting its evolutionary diversity, structural conservation, and distinct regulatory features and inform the potential roles of CmIAA genes in abiotic stress responses.