Genome-wide identification and comprehensive characterization of the Aux/IAA gene family in Cucurbita moschata and its responses analysis to abiotic stresses

Genome-Wide Identification of ZmIQM Gene Family and Their Roles in ABA-Mediated Seed Germination in Arabidopsis

Genome-wide identification of the expansin gene family in Rosa rugosa and overexpression of RrEXPA1 contributes to drought and salt stress tolerance in Arabidopsis.

The expansin (EXs) gene family plays a crucial role in the growth and development of various plants, as well as responses to biotic and abiotic stresses. However, genome-wide analysis of the EX gene family and their functions in drought and salt stress tolerance has not been examined in Rosa rugosa. In this study, a total of 30 RrEX genes were identified and located on seven different chromosomes. Phylogenetic analysis classified these genes into four subfamilies: EXPA (24 members), EXPB (3 members), EXLA (1 member), and EXLB (2 members). The average amino acid length was 269.17 aa, with isoelectric points ranging from 4.79 to 9.97. Most members exhibited high aliphatic indices and protein stability, suggesting their adaptability to diverse environments. The synteny analysis provided insights into the evolution of the EXs gene family in rose. Toxicity and autoactivation assays confirmed that BD-RrEXPA1 was non-toxic to yeast cells and lacked autoactivation activity, indicating its suitability for yeast two-hybrid screening. The transgenic Arabidopsis lines overexpressing RrEXPA1 improved seed germination and root length under abiotic stress. In addition, the overexpression lines showed reduced malondialdehyde (MDA) levels and increased chlorophyll content and superoxide dismutase (SOD) activity. These results suggest that RrEXPA1 may enhance stress tolerance by promoting root elongation and modulating physiological responses. This study provides important insights into the role of RrEXs in salt and drought stress and lays the foundation for further studies on the regulatory mechanisms of abiotic stress.

Genome-Wide Identification of the Maize TALE Gene Family and Their Expression Analysis Under Low-Phosphorus Response in Maize (Zea mays L.)

A comprehensive genome-wide identification and characterization of the maize TALE gene family were conducted using bioinformatics approaches, followed by an investigation of their transcriptional responses to low-phosphorus (LP) stress, providing valuable insights into the evolutionary characteristics and potential biological functions.

Genome-Wide Characterization of the PLATZ Gene Family in Medicago truncatula and Their Expression Profiles in Response to Abiotic Stress

A systematic analysis of the MtPLATZ gene family in M. truncatula is provided, offering a valuable reference for functional studies and genetic improvement of stress tolerance in legumes.

Evolutionary Analysis and Expression Profiling of the TIFY Gene Family in Banana Under Multiple Stresses and Functional Characterization of MaTIFY20 in Drought Tolerance

A genome-wide identification and comprehensive analysis of the MaTIFY gene family in Musa acuminata provides novel insights into the evolutionary dynamics and stress-responsive functions of banana TIFY genes and identifies candidate targets for molecular breeding to improve abiotic and biotic stress resilience in banana.

Genome-Wide Identification of the Soybean OXS3 Gene Family and Functional Characterization of GmOXS3-1 in Regulating Alkaline Tolerance

The Oxidative Stress 3 (OXS3) gene family encodes plant-specific proteins that play crucial roles in abiotic stress tolerance and chromatin remodeling. However, genome-wide identification and characterization of the OXS3 gene family in soybean have not been systematically conducted. Here, we identified 19 GmOXS3 genes from the soybean genome. Phylogenetic analysis assigned these genes to three subfamilies (I–III). Members within the same subfamily exhibited conserved motif compositions. The promoter regions of GmOXS3 genes contained various cis-acting regulatory elements associated with stress and phytohormone responses. Analysis of transcriptome data revealed that GmOXS3 genes exhibited different expression patterns in various organs. RT-qPCR further confirmed their differential expression under salt and alkaline stresses, with the most pronounced up-regulation observed for GmOXS3-1 and GmOXS3-14 under alkaline stress. Among them, GmOXS3-1 was further characterized, and it negatively regulates alkaline tolerance in soybean hairy roots. These results provide a foundation for elucidating GmOXS3-1-mediated alkaline stress signaling and highlight its potential as a breeding target for improving alkaline tolerance.