2026· Achievements and Prospects of Microbiology: Abstracts of the II International Scientific Conference· 0 citations
TL;DR
Overexpression of ADH6, HAA1, and GRE2 genes were performed and strains were characterized by increased in riboflavin production, characterized by increased in riboflavin production.
Abstract
Lignocellulosic biomass is a promising source of sugars, however, during hydrolysis the toxic compounds are released, that can inhibit the producing strain. For today there is no data on the genetic regulation in C. famata of tolerance to inhibitors (furfural, hydroxymethylfurfural (HMF), and acetic acid), as well as whether increased resistance would correlate with increased riboflavin production. Overexpression of ADH6 (coding for alcohol dehydrogenase), HAA1 (coding for transcription factor), and GRE2 (coding for aldehyde reductase) genes were performed for C. famata BRPI strain. Resulted strains were characterized by increased in riboflavin production. Keywords: riboflavin; Candida famata; yeast; riboflavin overproducers; lignocellulose hydrolysate; inhibitors.
A new factor of negative regulation of riboflavin biosynthesis in yeast C. famata was identified and encoded by the gene SEF2, which is focused on extension of the substrate range to produce the vitamin B2 such as whey and lignocellulose.
Dariya Fedorovych, K. Dmytruk, A. Sibirny· Achievements and Prospects o...· 0 citations
The non-conventional yeasts Ogataea polymorpha and Candida famata studied as hosts for the production of ethanol, L-lactic acid, and riboflavin from xylose, glycerol, and lignocellulosic hydrolysates provide a basis for further development of yeast-based production processes using lignocellulosic sugars and glycerol as...
A. Sibirny, Justyna Ruchała, Dominik Wojdyła et al.· Achievements and Prospects o...· 0 citations
Candida famata is a flavinogenic non-conventional yeast with a high capacity for riboflavin (vitamin B₂) production and represents a promising microbial cell factory. Glycerol, particularly crude glycerol generated as a by-product of biodiesel production, is an attractive low-cost carbon source for microbial fermentati...
Alicja Najdecka, A. Sibirny· Achievements and Prospects o...· 0 citations
Medium chain-methyl ketones are molecules with high energy content and various industrial applications. So far, biotechnological methyl ketone production was performed with glucose as a carbon source in minimal media, which is often not the best choice in terms of achieving a sustainable and cost-efficient bioprocess....
Carolin Grütering, C. K. Yamakawa, L. M. Blank et al.· Journal of Biotechnology· 0 citations
The utility of alternative carbon substrates as a tool for perturbing metabolism, redox balance, and fermentation product distributions is demonstrated to further the understanding of the metabolism of anaerobic, thermophilic bacteria such as C. thermocellum.
Isaiah D. Richardson, B. D. Sharma, Elizabeth Y. Ma et al.· bioRxiv· 0 citations
There was a synergistic effect for toxic stress resulting from the combined effect of the tested inhibitors, especially at medium and high doses of 5-hydroxymethylfurfural, furfural and vanillin.
J. Dróżdż-Afelt, B. Koim-Puchowska· Fungal Biology· 0 citations
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