A novel regulatory framework mediated by the BnNLA1-BnC07MYB3a module for controlling seed coat color in B. napus is revealed and will provide a new strategy for breeding high-quality B. napus cultivars.
Abstract
In rapeseed (Brassica napus L.), excessive accumulation of flavonoid pigments (anthocyanins/proanthocyanidins) compromises the nutrition, flavor, and commercial value. Therefore, reducing the accumulation of seed coat pigments is one of the main objectives in B. napus breeding. To elucidate the mechanisms affecting the genetic architecture of seed coat color, a genome-wide association study (GWAS) of seed coat color was conducted with a diverse group of 393 B. napus cultivars. NITROGEN LIMITATION ADAPTATION1 (NLA1) was identified as a previously unrecognized regulator that controls seed coat color and participates in flavonoid biosynthesis and accumulation in B. napus. Increasing evidence suggests that BnNLA1 (BnA09NLA1/BnC08NLA1) interacts with and ubiquitinates BnC07MYB3a, an R2R3-MYB-type transcription factor and a candidate regulator of the seed coat color in B. napus, and the lysine residue K164 of BnC07MYB3a is the key ubiquitination. Both the BnA09NLA1/BnC08NLA1 knock-out (KO) mutants and overexpressing BnC07MYB3a lines in B. napus exhibit lighter seed coat color indicating lower anthocyanin and proanthocyanidin accumulation compared with the wild-type plants. BnC07MYB3a also directly binds to the promoter of the TRANSPARENT TESTA 6 (BnTT6) and BANYULS (BnBAN), and represses their expression in B. napus. As expected, the expression levels of BnTT6 and BnBAN are significantly reduced in the BnA09NLA1/BnC08NLA1 KO mutants compared to the wild-type plants. Our findings reveal a novel regulatory framework mediated by the BnNLA1-BnC07MYB3a module for controlling seed coat color in B. napus and provide a new strategy for breeding high-quality B. napus cultivars.
A T2T rapeseed resource and a BnaWRKY44-BnaVPT1 module for breeding high-oil, yellow-seeded rapeseed are provided for breeding high-oil, yellow-seeded rapeseed.
Haijiang Liu, Yongheng Yuan, Kaijie Ye et al.· Cell Reports· 0 citations
Results indicate that BnaAOG1.A03 and BnaAOG1.C03 are not individually essential for silique and seed development in B. napus, providing a valuable case for functional analysis of homologous genes in polyploid crops.
Jiaxu Xiao, Xiao-Nan Guo, Aoli Liao et al.· Frontiers in Plant Science· 0 citations
The results demonstrate that BnaGRP3 may negatively modulate B. napus flowering time through modulating ABA signaling and circadian clock and identify BnaGRP3 as the candidate molecular breeding target for early-maturing rapeseed species.
Zhichao Mei, Xingru Xiang, Xin He et al.· Molecular breeding· 0 citations
As a major fungal pathogen of Brassica napus, Sclerotinia sclerotiorum causes significant yield reductions worldwide. Receptor-like proteins (RLPs) are essential components of plant immunity, but the functions of many RLPs in B. napus still remain unclear. In this study, three BnaSOBIR1-interacting leucine-rich repeat RLPs (LRR-RLPs), named BnaRLP-G13-2, BnaRLP-G13-3, and BnaRLP-G13-4, were identified by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Promoter analysis revealed diverse cis-acting elements involved in stress and phytohormone responses. Overexpression of these genes significantly improved resistance to S. sclerotiorum in both Arabidopsis thaliana and B. napus. Consistently, BnaRLP-G13-2/3/4 restored disease resistance and NLP-induced ROS production in the rlp23-1 mutant. Furthermore, BiFC assays suggested an association between BnaRLP-G13-2/3/4 and Ssnlp24SsNEP2-associated perception, although direct biochemical binding remains to be demonstrated. These findings advance the understanding of BnaSOBIR1-associated BnaRLP-G13-2/3/4-mediated immunity and provide new insights into enhancing disease resistance in oilseed crops.
LrMYB113 drives anthocyanin biosynthesis in Lycium ruthenicum by forming an MBW complex and directly activating LrDFR and LrANS promoters, providing a genetic target for enhancing flavonoid production. Lycium ruthenicum Murray (black goji berry), a Solanaceae medicinal plant, is valued for its high flavonoid content. However, the transcriptional regulation of flavonoid biosynthesis in L. ruthenicum remains unclear, hindering its pharmaceutical development. Here, we identified and characterized LrMYB113, an R2R3-MYB transcription factor, as a key regulator of anthocyanin biosynthesis in L. ruthenicum. Phylogenetic analysis grouped LrMYB113 into the anthocyanin-associated S6 subgroup of MYBs. Heterologous expression of LrMYB113 in tobacco induced pigment accumulation and upregulated anthocyanin pathway genes. LrMYB113 overexpression in L. ruthenicum hairy roots enhanced accumulation of four acylated anthocyanins and activated anthocyanin pathway genes. Yeast two-hybrid and bimolecular fluorescence complementation assays showed LrMYB113 interacts with bHLHs (LrJAF13/LrAN1b) and WD40 (LrAN11) to form an MBW complex. Promoter binding and transactivation assays demonstrated LrMYB113 directly binds to and activates LrDFR and LrANS promoters. Dual-luciferase assays showed LrMYB113 alone strongly activates LrDFR and LrANS promoters; MBW complexes enhanced activity compared to individual bHLH/WD40 but not to LrMYB113 alone. Our findings identify LrMYB113 as a critical regulator of anthocyanin biosynthesis in L. ruthenicum, shedding light on flavonoid molecular mechanisms and supporting genetic improvement for pharmaceutical use.
Tingting Li, Zihan Zhang, Jingjin Wang et al.· Plant Cell Reports· 0 citations
The genetic and molecular basis underlying anthocyanin accumulation in mei is revealed and a PmbHLH162-PmMYC2 regulatory module in which PmbHLH162 enhances PmMYC2-mediated activation of key anthocyanin biosynthetic genes is identified.
Bo-Da Liu, Zhi-Yuan Ma, Jing Pan et al.· Plant Physiology· 0 citations
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