TL;DR
The results demonstrate that BnaGRP3 may negatively modulate B. napus flowering time through modulating ABA signaling and circadian clock and identify BnaGRP3 as the candidate molecular breeding target for early-maturing rapeseed species.
It is shown that the loss of BnFLC genes significantly accelerates flowering, demonstrating that BnFLC genes regulate flowering in spring types independently of vernalization, and that these genes regulate flowering time developmentally in spring oilseed rape.
Flowering time is an essential complex quantitative agronomic trait in pak choi ( Brassica rapa ssp. chinensis ). It exerts decisive effects on crop yield, commercial quality and environmental adaptability throughout the whole cultivation cycle. Uncovering the key genes controlling flowering time is therefore critical for molecular genetic research and genetic improvement of pak choi. In the present work, we performed bulked segregant sequencing (BSA-seq) using an F 2 segregating population derived from a cross between late-flowering inbred line M1-006 and early-flowering line M2-001 to isolate and functionally characterize the flowering-related gene. Finally, a major-effect QTL governing flowering time was mapped to a 2.62 Mb genomic interval on chromosome A09, harboring a total of 440 annotated genes. Further gene function annotation pinpointed Brap0046500.1 (designated BcFPA ), an ortholog of the autonomous-pathway flowering regulator AtFPA from Arabidopsis thaliana , as the promising candidate gene within this target region, which was subsequently verified via polymorphic InDel molecular markers and genotype screening of F 2 individuals. Combined sequence comparison and functional assays demonstrated that a non-synonymous substitution in the coding region of BcFPA leads to delayed floral transition in pak choi. Consistently, transgenic complementation assays in Arabidopsis fpa-1 loss-of-function mutants verified that the wild-type BcFPA allele was sufficient to rescue the late-flowering phenotype and restore normal flowering. Moreover, overexpression of BcFPA in transgenic pak choi significantly accelerated flowering, further corroborating its positive regulatory role in floral initiation. This research systematically reveals the genetic mechanism responsible for natural flowering time variation in Brassica rapa. The identified BcFPA gene and its linked molecular markers can serve as important genetic resources for marker-assisted selective breeding, facilitating the breeding of new pak choi varieties with optimal flowering traits.
A T2T rapeseed resource and a BnaWRKY44-BnaVPT1 module for breeding high-oil, yellow-seeded rapeseed are provided for breeding high-oil, yellow-seeded rapeseed.
Background: Domain of Unknown Function 1645 (DUF1645) is a conserved but poorly characterized plant gene family whose evolutionary history and roles in stress adaptation remain unclear. We performed an integrated genomic, evolutionary, transcriptomic, and Quantitative Trait Locus (QTL) characterization of the DUF1645 family in rice (Oryza sativa). Methods and Results: We identified 14 intronless, non-redundant OsDUF1645 genes distributed across eight chromosomes. Phylogenetic and collinearity analyses suggested that family expansion within Poaceae involved ancestral segmental and localized tandem duplication events. Promoter analysis identified stress- and phytohormone-responsive cis-acting elements, including ABRE, MBS, and MeJA-associated motifs. Public transcriptome datasets revealed diverse OsDUF1645 expression patterns under abiotic and hormonal treatments. Integration with the Quantitative Trait Loci Annotation Rice Online (Q-TARO) QTLome identified physical co-localization of multiple OsDUF1645 loci with stress- and agronomic-trait QTLs, including salinity-, drought-, root architecture-, and water-deficit-associated regions. On Chromosome 1, OsDUF1645.1, OsDUF1645.2, OsDUF1645.3, and OsDUF1645.4 overlapped QTL intervals associated with salinity-related physiological traits, including Na+ uptake and Na+ balance, and drought-related root traits. On Chromosome 5, the tandemly arranged OsDUF1645.8, OsDUF1645.9, and OsDUF1645.10 co-localized with QTLs related to root architecture and water-deficit responses. qRT-PCR validation under salinity, osmotic stress, and cadmium exposure confirmed distinct stress-responsive expression profiles; OsDUF1645.6 exhibited broad multi-stress responsiveness, whereas OsDUF1645.3 was downregulated under several conditions. Conclusions: The OsDUF1645 family exhibits substantial functional diversification, supported by distinct regulatory architectures, expression profiles, and QTL associations. These findings provide a framework for prioritizing OsDUF1645 candidates for functional validation and their potential application in molecular breeding and development of climate-resilient rice cultivars.
Findings elucidate the molecular basis of flowering time in industrial hemp and provide valuable genomic resources for breeding broadly adapted, high-yield varieties.
Results indicate that BnaAOG1.A03 and BnaAOG1.C03 are not individually essential for silique and seed development in B. napus, providing a valuable case for functional analysis of homologous genes in polyploid crops.
We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.
