AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
A minimally invasive RNA labeling method featuring nucleotide- and position-selectivity, which solves the long-standing challenge of how to achieve accurate site-specific labeling of large RNAs with a least possible influence on folding and/or function.
TzG acts as an isomorphic and isofunctional substitute for guanosine, with fluorescence properties that are sensitive to local environmental changes that enable fluorescence‐based monitoring of RNA cleavage by deoxyribozymes and of site‐specific RNA methylation by a methyltransferase ribozyme.
We report the synthesis and testing of a new reagent for selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE). We report the synthesis and testing of a nitrone acyl imidazole that is capable of labelling RNA (DMImO-AI) for applications including in vivo click SHAPE (icSHAPE). The new reagent has a rea...
A miniaturized bioluminescent platform for RNA imaging that employs a compact RNA tag that assembles luciferase fragments upon binding of monomeric RNA-binding proteins (RNA lanterns) and establishes a miniaturized bioluminescent platform for imaging a broad range of RNA targets in living systems.
Pseudouridine (Ψ), often referred to as the “fifth nucleotide”, constitutes the most abundant post-transcriptional RNA modification and plays pivotal roles in regulating RNA structure and function. However, the precise mapping and functional investigation of Ψ remain challenging due to the lack of mild, nondestructiv...
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