Diverse routes to Cas3 activation in type I CRISPR-Cas immunity.

The global patent landscape of CRISPR–Cas applications in medicine

A CRISPR/Cas9 gene-editing platform for the rice leaffolder, Cnaphalocrocis medinalis.

A CRISPR/Cas9 toolkit was established for the rice leaffolder using entirely endogenous regulatory elements and provides a system for functional genomics in this pest.

Building CRISPR immunity: evolution and mechanisms of spacer acquisition.

CRISPR-Cas systems in prokaryotes serve as adaptive immune systems that neutralize phage infections through RNA-guided nucleases. Immunization is achieved during the adaptation stage through Cas1-Cas2 integrase-mediated insertion of short foreign DNA snippets, termed spacers, into a CRISPR array in the host genome. Thi...

EMERGING APPLICATIONS OF CRISPR–CAS9 BEYOND GENE EDITING

This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.

A steric hindrance-regulated autocatalytic CRISPR/Cas12a system for direct and amplification-free microRNA detection.

We constructed a CRISPR/Cas12a autocatalytic system based on a miRNA-triggered overhang blocker (OB) cleavage-recombination framework, in which Cas12a sequentially removed steric hindrance to initiate cyclic autocatalysis, enabling exponential signal amplification for visual miRNA detection without target pre-amplifica...

Generation of Elite Ideal Rice Germplasm via CRISPR/Cas9-Mediated Multiplex Targeted Gene Editing.

Gene editing of five key negative regulatory genes, GS3 , OsBADH2 , Xa13 , Bsr‐d1 and OsSULTR3;6 , generated an elite ideal‐type rice variety mk‐10 , which exhibits a longer grain shape, enhanced tolerance to heat and alkali stress, increased 2AP content, and simultaneous resistance to Xoo , rice blas...