Aug 2026· Plants· Vol 15· 0 citations· 35 references
Medicine
TL;DR
It is suggested that StuPPO9 confers drought resilience through a multi-layered network involving optimized carbon allocation, reinforced cell wall integrity, and enhanced ROS scavenging capacity.
Abstract
Drought stress is a primary abiotic constraint limiting potato productivity. While polyphenol oxidase (PPO) is known to participate in stress responses, the specific role of StuPPO9 in drought tolerance remains poorly understood. In this study, we generated StuPPO9-overexpressing (OE) and CRISPR/Cas9-mediated knockout (C4) lines in the potato cultivar ‘Atlantic’. Under sustained drought stress, OE lines exhibited significantly superior growth phenotypes compared to wild-type (WT) and C4 plants, characterized by increased leaf and root relative water content, root number and enhanced photosynthetic efficiency (Pn and Gs). OE plants also maintained lower levels of MDA and ROS through elevated antioxidant enzyme activities. Notably, transcriptomic analysis revealed that StuPPO9 triggers a global reprogramming of metabolic pathways. Key drought-responsive genes associated with phenylpropanoid biosynthesis (e.g., anthocyanin acyltransferase), terpenoid metabolism, and hormone signaling (e.g., HPt protein) were significantly upregulated in OE plants. These findings suggest that StuPPO9 confers drought resilience through a multi-layered network involving optimized carbon allocation, reinforced cell wall integrity, and enhanced ROS scavenging capacity. This study provides a promising genetic target and theoretical foundation for breeding drought-resistant potato varieties.
Drought stress is a major abiotic factor that adversely affects plant growth, development, and crop productivity. Lipoxygenases (LOXs) are key enzymes in the jasmonic acid (JA) biosynthetic pathway and play crucial roles in plant responses to environmental stresses. In this study, the ZmLOX2 gene was cloned from maize (Zea mays L.), and was found to encode a chloroplast-localized 13-lipoxygenase (13-LOX) protein. Expression analysis revealed that ZmLOX2 was predominantly expressed in leaves and was strongly induced under osmotic stress conditions. Compared with wild-type plants, transgenic Arabidopsis thaliana overexpressing ZmLOX2 exhibited significantly higher survival rates, enhanced growth performance, and improved root development under water deficit conditions. Physiological and biochemical analyses showed that the overexpression lines displayed higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), accompanied by significantly reduced malondialdehyde (MDA) content and reactive oxygen species (ROS) accumulation. Further investigation demonstrated that the expression levels of JA biosynthesis- and signaling-related genes, including ALLENE OXIDE SYNTHASE (AtAOS), 12-OXOPHYTODIENOATE REDUCTASE 3 (AtOPR3), MYC DOMAIN PROTEIN 2 (AtMYC2), and JASMONATE ZIM-DOMAIN 1 (AtJAZ1), were markedly upregulated in the transgenic lines. Protein–protein interaction analysis suggested that ZmLOX2 may interact with ALLENE OXIDE SYNTHASE 1 (AOS1), HYDROPEROXIDE LYASE 1 (HPL1), and ALPHA-DIOXYGENASE 1 (DOX1). Collectively, these results suggest that ZmLOX2 may contribute to plant adaptation to water limitation by regulating responses associated with JA, enhancing antioxidant defense, and maintaining ROS homeostasis.
Minghao Sun, Kechi Zhou, Tao Yu et al.· Plants· 0 citations
P phenotypic, physiological, and transcriptomic analyses were integrated to elucidate the drought adaptation mechanisms of a gamma-ray-induced mutant wheat line, PL6, alongside its wild-type parent, PL1, demonstrating an effective analytical framework for selection of high-confidence transcripts.
M. Hong, Ryu Jeong Kim, So Jin Park et al.· Agriculture· 0 citations
The potential of integrating the GmBAM-like 1 gene into plant breeding programs to develop cultivars with improved tolerance to water, salt, and osmotic stresses is demonstrated.
Fábia Guimarães-Dias, Lucas Leal Lima, A. C. Neves-Borges et al.· Genetics and Molecular Biolo...· 0 citations
Drought poses a serious challenge to global crop production. Identifying drought tolerance genes underpins the breeding of drought-resilient crop varieties. GATA transcription factors (TFs) are recognized as crucial modulators of plant abiotic stress responses, yet their specific functions in common wheat remain largely unexplored. In this study, we identified the TF TaGATA5, which encodes a protein containing a conserved ZnF_GATA domain across plant species. The expression of TaGATA5 was markedly induced by PEG, NaCl, and abscisic acid (ABA) treatments in wheat. Subcellular localization analysis showed that TaGATA5 was localized to the nucleus, and transactivation assays demonstrated its transcriptional activation ability. Compared with wild-type controls, TaGATA5 transgenic plants displayed improved growth performance and higher survival rates under drought stress by reducing water loss and maintaining higher relative water content. Moreover, TaGATA5 overexpression reduced reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content, accompanied by increased superoxide dismutase (SOD) activity. Conversely, TaGATA5 knockout mutants showed compromised drought tolerance, characterized by reduced growth performance, increased water loss, and lower survival rates. Yeast two-hybrid screening identified TaCOP9-2A as an interacting partner of TaGATA5. Both TaGATA5 and TaCOP9-2A function as positive regulators of drought tolerance. Notably, no negative effects on yield-related traits were observed in TaGATA5 transgenic plants under greenhouse conditions. Collectively, our findings elucidate the biological function of TaGATA5 in the wheat drought stress response and provide a valuable genetic resource for breeding drought-tolerant wheat varieties.
Jie Xiang, Jin-Ping Wang, Lijia Li et al.· Plant Science· 0 citations
This review synthesizes recent advances in elucidating the molecular and physiological mechanisms underlying drought tolerance in Vitis vinifera to provide an integrative conceptual framework to support sustainable viticulture in water-limited environments.
