Phytochemical Profiling and Preliminary Hepatoprotective Screening of Different Solvent Fractions of Elaeagnus parvifolia Fruit Extract Against Carbon Tetrachloride (CCl₄)–Induced Liver Injury in Mice
Aug 2026· Revista Brasileira de Farmacognosia· Vol 36· 0 citations· 95 references
TL;DR
Results confirm potent hepatoprotective effects linked to antioxidant and phenolic constituents, supporting E. parvifolia as a promising natural liver protectant against carbon tetrachloride–induced hepatotoxicity in mice.
The present study evaluated the hepatoprotective potential of Ipomoea pes-tigridis L. whole-plants extracts in paracetamol-induced hepatotoxic rat model. Sequential Soxhlet extraction yielded petroleum ether, ethyl acetate, and methanolic extracts, which were assessed at 200 mg/kg following of acute toxicity confirmation (2000 mg/kg). Paracetamol administration (2 g/kg) significantly elevated lipid peroxidation (TBARS: liver 2.99 ± 0.04 vs. control 1.44 ± 0.02 nmol MDA/g) and reduced antioxidant defences, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione (GSH) (p < 0.01). Pre-treatment with the ethyl acetate extract markedly restored antioxidant enzymes activities (e.g. hepatic SOD 5.83 ± 0.02 vs. 3.39 ± 0.03 in paracetamol group), and normalise total protein levels (7.21 ± 0.04 vs. 4.70 ± 0.02 g/dL), comparable to silymarin. Histopathology confirmed near-complete preservation of hepatic architecture. These findings demonstrate that the quercetin-rich ethyl acetate extract of Ipomoea pes-tigridis L. confers significant antioxidant-mediated hepatoprotection against paracetamol-induced liver injury.
It is indicated that combining papaya, neem, and garlic extracts may provide greater hepatoprotection than administering the individual extracts against DEN-induced liver injury, and supports further mechanistic and preclinical evaluation of this polyherbal formulation before its potential therapeutic application in liver-disease management.
Egeonu Us, Umaru I. J., Izam Ed et al.· African Journal of Clinical...· 0 citations
Liver diseases are a major global health burden, and oxidative stress plays
a key role in the development of hepatocellular injury. Mimusops elengi L. fruits contain bioactive
phytochemicals with reported antioxidant properties. This study aimed to evaluate the hepatoprotective
activity of aqueous Mimusops elengi fruit extract against carbon tetrachloride (CCl4)-induced
liver injury in Wistar rats.
Male Wistar rats were randomly allocated into five groups (n = 5). Hepatic injury was
induced using CCl4 (1 mL/kg, i.p.) for 15 days. Animals received Mimusops elengi fruit extract (200
and 400 mg/kg, orally) or silymarin (25 mg/kg). Serum ALT, AST, and catalase activity were evaluated,
followed by histopathological examination of liver tissues.
CCl4 administration significantly increased serum ALT and AST levels while reducing catalase
activity, indicating severe hepatic injury. Treatment with Mimusops elengi fruit extract significantly
reduced liver enzyme levels and improved antioxidant status in a dose-dependent manner.
Histopathological examination demonstrated preservation of hepatic architecture, reduced necrosis
and decreased inflammatory changes, particularly at 400 mg/kg.
The hepatopr tective activity observed may be attributed to the antioxidant and membrane-
stabilizing properties of flavonoids, tannins, and saponins present in Mimusops elengi fruits.
The biochemical and histopathological findings collectively support the traditional medicinal use of
this plant for liver protection and suggest its potential as a natural hepatoprotective agent.
Mimusops elengi fruit extract exhibits significant hepatoprotective effects against
CCl4-induced liver injury, likely mediated through antioxidant mechanisms. These findings support
its potential as a natural therapeutic agent, although further studies are required to elucidate its active
constituents and molecular pathways.
Nisha Chandila, Suresh Kumar, Manoj Kashyap· The International Journal of...· 0 citations
Malaria remains one of the most devastating tropical diseases, responsible for significant morbidity and mortality, particularly in developing countries. This study aimed to evaluate the antimalarial activity, phytochemical composition, metal content, and haematological effects of Morinda citrifolia leaf extracts in order to establish their therapeutic potential and safety profile. Leaf extracts were fractionated using vacuum liquid chromatography, and n-hexane, ethyl acetate, and methanol fractions were assessed for in vivo antiplasmodial activity against Plasmodium berghei in infected mice at doses of 200, 400, and 800 mg/kg. Additionally, metal analysis, haematological studies on red and white blood cells, and total flavonoid content determination were carried out using standard analytical methods The antimalarial evaluation revealed dose-dependent chemosuppression, with values increasing from 13.68–36.19% at 200 mg/kg to 21.06–45.51% at 800 mg/kg across the extracts, indicating significant antiplasmodial activity, although lower than that of the standard drug. Metal analysis showed the presence of essential elements such as iron (2.00 mg/kg), zinc (0.88 mg/kg), manganese (2.25 mg/kg), and chromium (0.195 mg/kg), with lead (0.46 mg/kg) remaining within FAO/WHO permissible limits, suggesting the extract is relatively safe. Haematological studies demonstrated significant increases in red blood cell count, hemoglobin concentration, and hematocrit levels in treated rats, indicating positive effect on erythropoiesis. The methanol leaf extract exhibited high total flavonoid content of 86.12 mg/g quercetin equivalent, confirming the plant’s rich phytochemical profile.
Matthew Edekin Oni, Emmanuel Ovwero, K. Ogbeide· FUDMA Journal of Sciences· 0 citations
Introduction: Indigofera stenophylla Guil. and Perr. (Fabaceae) is a medicinal plant used in traditional medicine for the treatment of diseases. This study aimed to isolate phytoconstituent(s) and establish the hepatoprotective activity of the methanol aerial parts extract of Indigofera stenophylla (MAEIS). Methods: The plant material of 1500 g was extracted with 70% methanol using a maceration method, and the extract was partitioned respectively into hexane and chloroform fractions (HF and CF). The HF was subjected to column chromatographic techniques to afford 5 mg of compound AR1 characterized using 1H and 13C-NMR. A single dose of 7,12 Dimethylbenz(a)anthracene was administered orally at 30 mg/kg body weight, followed by 28 days treatment with graded doses of MAEIS and Silymarin. Blood samples were collected and analyzed for Aspartate Amino Transaminase (ASP), Alanine Amino Transaminase (ALT) and Total Protein (TP) assays. The livers were harvested for histopathological examination. Results: The isolated compound AR1 was elucidated as lup-20(29)-en-3-ol (lupeol). The MAEIS group at dose of 600 mg/kg and silymarin 200 mg/kg body weight respectively lowered the levels of AST and ALT significantly (p<0.05) indicating a reduction in liver damage. The MAEIS increased the total protein levels and normal histopathological profile of the liver tissue. Conclusion: This is the first isolation of lupeol from MAEIS. MAEIS provides a scientific basis for its ethnomedicinal uses and demonstrated significant hepatoprotective activity.
A. Sanusi, Ngaitad Stsnislaus Njinga, Sa.ad Toyin Abdullahi et al.· The Journal of pharmacy· 0 citations
Sodium benzoate is one of the most widely used food preservatives in the food and beverage industry due to its effectiveness in inhibiting microbial growth. However, excessive and prolonged consumption of sodium benzoate may induce adverse health effects, including gastrointestinal disturbances, neurological disorders, and liver damage resulting from oxidative stress. Natural medicinal plants with antioxidant properties have attracted considerable attention as potential therapeutic agents against chemical-induced organ toxicity. Keji Beling leaves (Strobilanthes crispa L. Blume) are traditionally utilized in Indonesia for various medicinal purposes and are known to contain bioactive compounds such as flavonoids, polyphenols, tannins, and saponins that exhibit hepatoprotective activity. This study aimed to evaluate the effect of ethanol extract of keji beling leaves on liver histology and liver physiological function, as indicated by serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) levels, in sodium benzoate-induced white rats (Rattus norvegicus L.). The experiment employed a Completely Randomised Design with five treatment groups at different extract dosages. Data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test at a 95% confidence level. The results demonstrated that sodium benzoate administration significantly induced liver histopathological alterations characterized by cellular degeneration, inflammation, and necrosis, accompanied by elevated SGPT and SGOT levels. Treatment with the ethanol extract of keji beling leaves significantly improved liver histology and reduced liver enzyme levels compared with the sodium benzoate-induced group. The hepatoprotective effect was dose-dependent, with the dose of 500 mg/kg body weight showing the most effective recovery of liver tissue and normalization of SGPT and SGOT levels. These findings suggest that the ethanol extract of Strobilanthes crispa leaves has promising potential as a natural hepatoprotective agent against sodium benzoate-induced liver injury.
Unknown authors· JURNAL PIJAR MIPA· 0 citations
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