Refined Mapping of Endothelial Cells, Brain Pericytes and Astrocytes Proteomes via Subcellular Fractionation and DDA Mass Spectrometry
TL;DR
An integrative proteomic analysis of endothelial cells, pericytes, and astrocytes—the three main cell types orchestrating vascular and cerebral metabolism and functions—is presented, using whole-cell lysates and subcellular fractionation combined with data-dependent acquisition mass spectrometry.
Abstract
Simple Summary This study presents an integrative proteomic analysis of endothelial cells, pericytes, and astrocytes—the three main cell types orchestrating vascular and cerebral metabolism and functions—using whole-cell lysates and subcellular fractionation combined with data-dependent acquisition mass spectrometry. Subcellular fractionation enhanced proteome coverage by up to 93%, especially for membrane-associated and insoluble proteins, revealing sharper cell-type-specific functional signatures. However, sampling strategy and cell differentiation status were shown to influence protein detection and interpretation of specificity. By merging data across approaches, we generated reference proteomes for each cell type, offering valuable resources for neurovascular research and biomarker discovery.