Caracterização morfofisiológica, molecular e funcional de Mycobacterium agroflorensis com potencial para o desenvolvimento de bioinsumos e promoção do crescimento vegetal
Bacterial inoculation improved plant development and demonstrated promising potential for application in sustainable agricultural systems, highlighting the biotechnological potential of M. agroflorensis as a candidate for the development of agricultural bioinputs, contributing to low-carbon agriculture and advancing research in agricultural microbiology.
Abstract
The growing demand for sustainable agricultural systems has stimulated the development of microbial bioinputs capable of reducing dependence on mineral fertilizers while improving the efficient use of natural resources. In this context, the prospecting and characterization of plant growth-promoting bacteria represent a promising strategy to increase agricultural productivity and support environmental sustainability. This study aimed to perform the morphophysiological, molecular, and functional characterization of the experimental strain Mycobacterium agroflorensis, evaluating its potential for application as an agricultural bioinput. The research included macroscopic and microscopic characterization, cultivation on Middlebrook 7H10 medium, assessment of bacterial growth under different temperature and pH conditions, molecular identification based on 16S rRNA gene sequencing, and investigation of plant growth-promoting mechanisms, including phytohormone production, phosphate solubilization, siderophore production, and genes associated with plant–bacteria interaction. Agronomic assays were also conducted under controlled environmental conditions, combined with Internet of Things (IoT)-based environmental monitoring, to evaluate bacterial inoculation in different crop species. The results demonstrated that M. agroflorensis exhibited high phenotypic stability, optimal growth at 30 °C and pH 7.0, morphological characteristics consistent with the genus Mycobacterium, and functional traits associated with plant growth promotion. Bacterial inoculation improved plant development and demonstrated promising potential for application in sustainable agricultural systems. These findings highlight the biotechnological potential of M. agroflorensis as a candidate for the development of agricultural bioinputs, contributing to low-carbon agriculture and advancing research in agricultural microbiology.
The biotechnological potential of Mycobacterium agroflorensis as a promising microbial bioinput for sustainable agriculture is highlighted, supporting future studies aimed at field validation and the development of innovative biofertilizers for environmentally responsible crop production.
Luís Dias Ferreira Soares, Zilmar Timóteo Soares, Aarão Felipe Ataídes Lima et al.· ARACÊ· 0 citations
Megathyrsus maximus (Guinea grass) is an important forage species for livestock production, whose productivity has been adversely affected by the excessive use of chemical fertilizers. The objective of this study was to isolate and characterize plant growth-promoting bacteria (PGPB) associated with the rhizosphere of M. maximus. Rhizosphere samples were collected and processed for bacterial isolation, followed by microbiological, biochemical, and molecular characterization. Plant growth-promoting traits were assessed through urease activity, phosphate solubilization, catalase activity, amylolytic activity, and indole-3-acetic acid (IAA) production. In addition, in vitro assays were conducted to evaluate seed germination, root length, leaf length, stem length, and fresh plant weight. The results demonstrated the successful isolation and identification of rhizosphere-associated bacteria with plant growth-promoting potential, regardless of their geographic origin, revealing considerable genetic diversity among the isolates. The best-performing strains were molecularly identified as Azospirillum lipoferum, Rhizobium etli, Klebsiella oxytoca, and Serratia marcescens. These findings highlight the potential of these bacterial species as biofertilizers to enhance the establishment, growth, and productivity of M. maximus, providing a sustainable alternative to reduce dependence on chemical fertilizers and supporting the development of more efficient and environmentally responsible livestock production systems under tropical conditions
Juan Pablo Avellaneda Vázquez, Karina Gabriela Martinez Lara, Karelys Edith Hidalgo Mendoza et al.· Multidisciplinary Collaborat...· 0 citations
This systematic review analyzed the chemical characterization, bioactive properties, and agro-industrial potential of wild berries and fruits. Following the PRISMA 2020 methodology, 34 scientific articles published between 2019 and 2025 were selected from the ScienceDirect, MDPI, and SciELO databases. The reviewed studies demonstrated that various species of wild berries and fruits contain high concentrations of phenolic compounds, flavonoids, and anthocyanins, which are associated with significant antioxidant, anti-inflammatory, antimicrobial, and antitumor activities. Furthermore, the potential of technologies such as microencapsulation was identified as a means to enhance the stability of sensitive bioactive compounds and facilitate their incorporation into functional foods, nutraceuticals, and other agro-industrial applications. Similarly, the valorization of by-products—such as pomace and bagasse—represents a sustainable strategy within circular economy models. The results highlight the importance of wild berries and fruits as promising sources of functional ingredients with the potential for developing high-value-added products.
Christell Marjorie Chero Calcina, Gina De La Cruz-Calderón, Estefani Raquel Espinoza Bovis et al.· KANYÚ· 0 citations
The objective was to characterize the species’ biotechnological potential and its phenotypes, with an emphasis on the morphophysiology and biochemistry of fruits, seeds, and germination, as well as the antioxidant activity of the leaf essential oil.
This research analyzed the production and application of a sustainable biofertilizer made with native Caatinga species, such as *Mimosa tenuiflora* (jurema-preta) and *Spondias purpurea* (seriguela), as an agroecological alternative for family farming in the semi-arid region. The study was developed in Granjeiro, Ceará, in response to the problem of excessive use of chemical fertilizers and pesticides, which degrade the soil and increase production costs. The aim was to evaluate whether a bio-input produced with local species could reduce dependence on synthetic inputs. The objective was to analyze its efficiency in the growth of crops such as beans, corn, tomatoes, lettuce, and coriander. The methodology involved leaf fermentation, controlled application, and monitoring for thirty days. The results indicated greater vegetative vigor, better root development, and greater resistance to water stress. The research reinforces the value of Caatinga resources and integrates traditional and scientific knowledge. It concludes that the biofertilizer presents sustainable, economic, and environmental potential to strengthen family farming in the semi-arid region.
Lucas Melo dos Santos, José Eduardo Melo dos Santos, Márcio de Oliveira Lima et al.· International Journal Semiar...· 0 citations
The cultivation of Musa spp. is facing increasing losses due to erratic rainfall in tropical growing regions, making it necessary to explore biological alternatives that reduce dependence on irrigation without compromising seedling development. The effect of a consortium of plant-growth-promoting rhizobacteria, capable of forming biofilms in the rhizosphere, on the morphophysiological development of Williams banana seedlings subjected to three levels of water availability during the nursery phase was evaluated. A completely randomised design was used, comprising six treatments and three replicates, with assessments of height, pseudostem diameter, number of leaves, relative chlorophyll content and fresh root weight. The treatments without bacterial inoculation consistently outperformed the inoculated treatments for all variables with statistical significance, achieving the highest performance under the least water-limiting conditions. Inoculation did not induce any detectable improvements in any of the morphological or photosynthetic variables assessed. These results suggest that, under the conditions tested, seedling development depended primarily on intrinsic physiological mechanisms in response to water deficit, rather than on the activity of the bacterial consortium.
Maholy Rocio Loza-Ormaza, Fabricio Rolando Fuentes-Núñez, Anthony Lizandro Tubun-Vargas et al.· Revista Científica Ciencia y...· 0 citations
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