Selection and validation of optimal housekeeping gene through RNA-sequence and qRT-PCR analysis in rat testis tissues and Sertoli cells under mangancism and Coridius chinensis extracts rescue
This study demonstrates that PPIA may serve as the optimal HKG in rat testicular tissues and Sertoli cells under various pathological conditions, providing valuable reference data for functional gene expression studies related to testicular diseases in mammals.
Reverse transcription quantitative real-time PCR (RT-qPCR) is widely used to quantify gene expression, but its accuracy depends on appropriate normalization using stable reference genes (RGs). Because methamphetamine (METH) exposure induces widespread transcriptional changes, conventional housekeeping genes may not rem...
Sang-Je Park, Se-Hee Choe, Hyeon-Mu Cho et al.· Neurotoxicology· 0 citations
Real-time quantitative PCR (qPCR) is a key technique for elucidating the mechanisms underlying environmental stress responses in aquatic animals, and its accuracy relies heavily on stable reference genes for data normalization. In this study, histopathological examination revealed alterations in the gill, hepatopancrea...
Xiu-Dan Yuan, Xing Tian, Yuan-Yuan Liu et al.· Biology· 0 citations
Quantitative real-time PCR (qRT-PCR) is a widely used technique for quantifying gene expression. However, accurate data normalization requires stable reference genes that exhibit constant expression levels across different experimental conditions. To date, no suitable reference genes have been validated for atemoya fru...
Xue-Yu Zhang, Q. Gao, Ying Zhou et al.· International Journal of Mol...· 0 citations
Simple Summary Gene expression analysis is essential for evaluating feline mesenchymal stem cells (MSCs). While quantitative real-time PCR (qRT-PCR) is a standard tool for this purpose, its accuracy depends on selecting stable reference genes for data normalization. The routine use of traditional reference genes withou...
Rubel Miah, Sang-Yun Lee, S. Song et al.· Veterinary Sciences· 0 citations
This study provides a set of reliable, con-dition-adaptive RGs for P. quinquefolius and establishes a robust molecular tool for future studies on ginsenoside biosynthesis and stress response mechanisms in American ginseng.
Fang-Zhou Zhao, Jia-Xin Zhang, Yu-Juan Zhang et al.· 0 citations
Quantitative real-time PCR (qRT-PCR) is a dominant technique for gene expression analysis in medicinal plants, and accurate qRT-PCR data normalization strictly relies on stably expressed reference genes (RGs). However, systematically validated RGs for Panax quinquefolius L. across diverse tissues, abiotic stresses, and...