Identi fi cation of Reference Genes and Their Application in Analysis of Ginsenoside Biosynthesis Genes in American Ginseng Under Abiotic Stresses and Hormone Treatments
This study provides a set of reliable, con-dition-adaptive RGs for P. quinquefolius and establishes a robust molecular tool for future studies on ginsenoside biosynthesis and stress response mechanisms in American ginseng.
Quantitative real-time PCR (qRT-PCR) is a dominant technique for gene expression analysis in medicinal plants, and accurate qRT-PCR data normalization strictly relies on stably expressed reference genes (RGs). However, systematically validated RGs for Panax quinquefolius L. across diverse tissues, abiotic stresses, and...
Quantitative real-time PCR (qRT-PCR) is a widely used technique for quantifying gene expression. However, accurate data normalization requires stable reference genes that exhibit constant expression levels across different experimental conditions. To date, no suitable reference genes have been validated for atemoya fru...
Xue-Yu Zhang, Q. Gao, Ying Zhou et al.· International Journal of Mol...· 0 citations
Gene expression profiling plays a pivotal role in elucidating molecular regulatory networks and identifying functionally critical genes. However, the accuracy and reliability of quantitative real-time PCR (qPCR) assays fundamentally depend on the selection of stable expressed reference genes. Ilex verticillata, an econ...
Selecting and validating appropriate reference genes is a prerequisite for accurate gene expression analysis by means of quantitative real-time PCR (qRT-PCR), particularly in plant species with limited molecular research. Elsholtzia stauntonii is an economically important ornamental, medicinal, and aromatic plant. Alth...
Miao-Miao Liu, Xiang-Yu Lin, Xiu-Mei Zhou· Current Issues in Molecular...· 0 citations
Sugarcane and energy cane (Saccharum spp. hybrids) yields are significantly threatened by smut disease, caused by the fungus Sporisorium scitamineum. Gene expression studies are essential for understanding molecular responses in this pathosystem; however, reference gene stability may vary among genotypes, plant organs,...
Laísa M. Rocha, D. F. Contiliani, Vanessa Thomé et al.· International Journal of Mol...· 0 citations
A comprehensive genome-wide analysis to characterize the 19-member OsDMP family in rice provides a robust framework for understanding OsDMP evolution and expression regulation, revealing transcriptional responsiveness to abiotic stress and providing candidate genes for future functional validation and application in br...