RNAi-mediated knockdown of vitellogenin genes disrupts ovary development and alters ovarian bacterial composition in Zeugodacus tau (Diptera: Tephritidae).
Aug 2026· Journal of Economic Entomology· 0 citations
Medicine
TL;DR
Findings elucidate the important function of Vgs in ovary development and provide insights into the association between insect Vg genes and the ovarian bacteria, thereby establishing a groundwork for subsequent investigations on the molecular mechanisms underlying their interaction.
The oriental fruit fly Bactrocera dorsalis is a globally invasive pest with increasing insecticide resistance that threatens sustainable crop production. ATP-binding cassette (ABC) transporters are key mediators of xenobiotic detoxification, but the molecular and regulatory basis of ABCC-type transporters in this species remains unclear. Here, we present the first systematic reannotation and functional analysis of the ABCC gene family in B. dorsalis and identify a novel post-transcriptional regulatory pathway involving miR-980. Using updated genomic and transcriptomic resources, we accurately annotated eight BdABCC genes with conserved nucleotide-binding (NBD) and transmembrane domains (TMD) and strong phylogenetic conservation with dipteran homologs. Spatiotemporal expression profiling showed constitutive expression across development, elevated transcription in the midgut, Malpighian tubules and fat body, and transcriptional responsiveness of several members, particularly BdABCC1, BdABCC2, BdABCC4, and BdABCC6, to multiple insecticides. RNA interference and MK-571 inhibition demonstrated that BdABCC1 contributes to avermectin tolerance. Furthermore, luciferase reporter assays, RNA pull-down and fluorescence in situ hybridization confirmed that miR-980 directly targets the BdABCC1 coding sequence, with miR-980 overexpression suppressing BdABCC1 and increasing avermectin-induced mortality. Together, these findings identify a previously uncharacterized miR-980-BdABCC1 regulatory axis that modulates avermectin tolerance and highlights post-transcriptional regulation of ABC transporters as a potential molecular target for future RNAi-based pest management strategies.
Hou-Ji Song, Lin Wang, Wei Zhao et al.· Insect Biochemistry and Mole...· 1 citation
DmaxVgR gene silencing disrupted follicular architecture and reduced vitellogenin uptake by the oocytes, resulting in increased vitellogenin levels in the hemolymph suggest a feedback mechanism regulating YPP production.
F. O. Ramos, Jimena Leyria, M. Nouzová et al.· Insect Biochemistry and Mole...· 0 citations
Findings establish BrBGLU10 as an important regulator of pollen development and a potential target for fertility-related applications via gene editing in B. rapa and related Brassica crops.
Ying Huang, Shanxin Zhong, Tian-Ci Hu et al.· Plants· 0 citations
Background: Siraitia grosvenorii (monk fruit) is a dioecious medicinal crop native to southern China, yet its terpene synthase (TPS) gene family and the molecular basis of floral sexual dimorphism remain unexplored. Methods: Genome-wide identification of the TPS gene family was performed using HMMER and BLAST-based approaches. Phylogenetic classification, gene structure and conserved motif characterization, and comparative synteny analyses were conducted. Transcriptome data from leaves and fruits at different developmental stages were analyzed for tissue-specific expression profiling. Promoter cis-element analysis, protein–protein interaction network prediction, and molecular docking were performed to characterize the fruit-specific candidate SgTPS49. Results: A total of 58 SgTPS genes were identified and classified into six subfamilies, with TPS-a and TPS-b comprising 72.4% of the family. Approximately 88% of SgTPS genes arose from lineage-specific tandem duplication. Female flowers exhibited monoterpene-dominant scents and smaller corollas, whereas male flowers displayed a mid-morning sesquiterpene burst and greater morphological variation. SgTPS49 was specifically upregulated at 20 days post-pollination and possessed a unique promoter architecture devoid of classical hormone-responsive elements. Molecular docking supported its annotation as a putative monoterpene synthase with favorable GPP binding. Conclusions: This study provides the first comprehensive genomic resource for the SgTPS family in S. grosvenorii, reveals significant sexual dimorphism in floral traits, and identifies SgTPS49 as a key candidate for future functional validation.
Xiao-Zhen Zhu, Qifeng Lu, Changqi Liu et al.· Genes· 0 citations
The findings show that tick vitellogenesis pathways contribute to Rb regulation during reproduction and identify the I. scapularis-Rb system as a useful model for studying host control of obligate endosymbionts.
Dattatray V. Sawant, Yating Dong, Haritha Katasani et al.· bioRxiv· 0 citations
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