An On-Demand Nanodisc Platform for Reconstitution of Functional Membrane Proteins into Model and Living Membranes

ORB-TXTL: cell-free expression of membrane proteins on lipid bilayer-coated beads

On-bead Reconstitution into Bilayers via Cell-free Transcription and Translation (ORB-TXTL), a platform that uses compositionally tunable lipid bilayer-coated silica beads as scaffolds for cell-free synthesized interacting and integral membrane proteins, is developed.

An Automated HDX−MS Platform for Characterization of Membrane Proteins within Biological Membranes

This work presents the first fully automated HDX−MS platform incorporating a two-stage delipidation workflow and underscores the importance of studying membrane proteins in native lipid environments.

Extracellular vesicle fusion-mediated compartmentalized membrane protein reconstitution toward intracellular signaling coupling.

A novel reconstitution platform is developed that exploits the membrane fusion capability of extracellular vesicles to incorporate membrane proteins into planar lipid bilayers with high efficiency and preferential orientation and provides a versatile foundation for membrane protein analysis, drug discovery, and the eng...

Membrane Formulation Impacts Vesicle-Based Artificial Cell Functions

The study underscores the importance of membrane engineering as a powerful strategy for advancing the rational development of artificial cells with functional capabilities by identifying the limiting factor that underlies the observed differences in protein expression between membrane formulations: the efficiency of ma...

Bottom-up engineering of EV-mimetic vesicles reveals selective protein loading and modulates cellular uptake and biodistribution

Designing nature's nanocarriers: advances and challenges in functionalizing bacterial extracellular vesicles.

This review examines luminal targeting strategies (secretion pathway-mediated loading, lipoprotein anchoring, and membrane scaffold fusions), surface display platforms, and genetic yield-enhancement mechanisms to advance bEVs toward clinically viable, programmable delivery systems.