iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones
Abstract
This Zenodo deposit contains a publicly available description of the Dataset: Title: "iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones". Description: Targeted DNA sequencing data for the iSCORE-PD isogenic hESC clone collection, encompassing three complementary analyses: (1) NGS genotyping of 53 mutant clones across 11 PD-linked genes via amplicon sequencing on Illumina iSeq 100 with CRISPResso2 analysis, (2) zygosity testing of a subset of clones in 6 genes via SNP-PCR (NGS or Sanger) to assess zygosity near CRISPR edit sites, and (3) Sanger sequencing of the wild-type allele in 5 DJ1/PARK7 heterozygous clones. This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset
