It is demonstrated that CRISPR can be employed to edit Acanthamoeba genes using a knock-in approach and serves as a foundation to further develop A. castellanii as a model system with which to study diverse questions in cell and molecular biology, biochemistry and evolution.
Cpf1-based genome editing tools were developed for N. punctiforme, and a single-step cloning strategy was devised, allowing for rapid assembly of editing plasmids, and improved conjugation protocols for genetic transfer from E. coli to N. punctiforme were implemented.
J. Ryder, Soohan Woo, Ailea A. Blahm et al.· bioRxiv· 0 citations
Compared with conventional homologous recombination, the CRISPR-Cas9 system substantially improved gene disruption efficiency, thereby overcoming a major limitation in the genetic manipulation of lichen-forming fungi.
Ze-Yi Wang, Niu-Niu Wang, Hai-Yu Zhang et al.· Journal of Fungi· 0 citations
It is demonstrated that Avr4 does not explain the resistance of Calcutta 4, suggesting that resistance is instead triggered by the recognition of other hitherto unknown effectors.
Maikel B. F. Steentjes, Gregory Ashe, Patricia Schöppl et al.· bioRxiv· 0 citations
An inducible genome-editing framework exploiting the endogenous Cas9 system of Apilactobacillus kunkeei, a key member of the honeybee microbiota, is established and the toolkit available for harnessing endogenous CRISPR-Cas systems in genetically recalcitrant, non-model gram-positive bacteria is expanded.
Mahesh S. Iyer, Erik Hagström, Kristina Näslund et al.· Applied and Environmental Mi...· 0 citations
