AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
Komagataella phaffii (formerly Pichia pastoris) has emerged as an indispensable eukaryotic expression host for the production of industrial enzymes, food proteins, and therapeutic proteins owing to its Generally Recognized as Safe (GRAS) status, efficient protein secretion capacity, eukaryotic post-translational modifi...
Efficient biosynthesis of 1,3-PDO, a key bio-based chemical, depends on precise regulation of the host metabolic network. In this study, a heterologous CRISPR-Cas12a genome editing system was established and systematically optimized in Klebsiella pneumoniae, enabling efficient and stable genome editing (75-100% efficie...
Metabolic engineering of Talaromyces pinophilus through promoter optimization, multicopy integration, and protease deletion enables efficient α-amylase production from lignocellulosic biomass, achieving 26 712 U/mL in bioreactor fermentation.
It is demonstrated that maximizing recombinant protein production requires optimization of both yield and structural quality and establish complementary strategies for improving secreted protein expression in Pichia.
Lactosucrose is a functional trisaccharide with prebiotic properties and growing potential in food and agricultural applications; however, its industrial production is limited by low conversion efficiency and high downstream processing costs. In this study, a whole-cell biocatalyst was developed by displaying β-galacto...
Acid proteases can improve nitrogen availability during ethanol fermentation by hydrolysing feedstock proteins, but their catalytic performance at relatively low temperatures may be limited. In this study, an acid protease-producing Rhodotorula mucilaginosa strain, TSM-6, was isolated from mangrove sediment, and an aci...
We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.