Complete chloroplast genome and phylogenetic analysis of Amorphophallus ochroleucus Hett. & V.D.Nguyen 2001 (Araceae)

The complete chloroplast genome and phylogenetic analysis of Urceola huaitingii (Chun & Tsiang) D. J. Middleton 1994 (Apocynaceae)

Abstract Urceola huaitingii (Chun & Tsiang) D. J. Middleton 1994, a member of the Apocynaceae family, is widely distributed across southern and southwestern China and has been traditionally used in folk medicine to treat hemiplegia and paralysis. In this study, we report the first complete chloroplast genome of U. huaitingii and perform phylogenetic analysis with 30 related species within the Apocynaceae. The chloroplast genome of U. huaitingii is 155,182 bp in length and has a GC content of 38.11%. It displays a typical quadripartite structure, consisting of a large single-copy (LSC) region of 85,254 bp, a small single-copy (SSC) region of 18,242 bp, and two inverted repeat (IR) regions of 25,843 bp each. A total of 111 unique genes were annotated, including 77 protein-coding genes, 30 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes. Phylogenetic analysis revealed that U. huaitingii is closely related to the genera Aganosma, Trachelospermum, and Amalocalyx. This study provides the first chloroplast genomic resource for the genus Urceola, laying a foundation for future investigations into its evolutionary relationships. It also contributes to future molecular and phylogenetic studies within the Apocynaceae family.

The first complete chloroplast genome and phylogenetic analysis of Veratrum dolichopetalum O.Loes. (Melanthiaceae)

Abstract Veratrum dolichopetalum O.Loes. (1926) is a perennial herbaceous plant in the family Melanthiaceae that is geographically restricted to northern Korea and the Ussuri River basin of Russia. Here, we present the first complete chloroplast genome of V. dolichopetalum, which is 153,673 bp in length and contains 82 protein-coding gene copies, 38 tRNA genes, and 8 rRNA genes. The genome exhibits the typical quadripartite structure, comprising a large single copy region (83,352 bp), a small single copy region (17,603 bp), and a pair of inverted repeats (26,359 bp each). Phylogenetic analysis based on 77 orthologous chloroplast protein-coding genes indicated that V. dolichopetalum is closely related to V. oxysepalum Turcz. The complete chloroplast genome reported here provides essential genomic resources for further evolutionary studies of Veratrum.

The complete mitochondrial genome of Acanthaspis geniculata (Hsiao, 1976) (Hemiptera: Reduviidae) and its phylogenetic analyses

Abstract The genus Acanthaspis (Hemiptera: Reduviidae) comprises predatory insects of ecological importance, yet mitochondrial genomic resources for this group remain limited. Here, we present the complete mitochondrial genome of Acanthaspis geniculata (Hsiao, 1976). The mitogenome is 16,226 bp in length and contains the standard set of 37 mitochondrial genes (13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes), along with a control region (D-loop). The nucleotide composition is A (40.4%), T (29.6%), C (17.9%), and G (12.1%), with an overall AT content of 70.0%. Phylogenetic analyses based on partitioned mitochondrial datasets strongly support the monophyly of the subfamily Reduviinae and reveal that A. geniculata forms a close sister-group relationship with A. ruficeps and A. pedestris. This study provides a fundamental mitogenomic resource for future evolutionary and systematic studies of the Reduviidae.

Plastome characterization of Embelia ribes Burm. f. (Myrsinoideae: Primulaceae): comparative analysis and phylogenetic inference

The first complete chloroplast genome of Calamus erectus (Arecaceae): Phylogenetic placement and divergence time estimation

The complete plastome of C. erectus is characterized, providing the first complete cp genome resource for C. erectus, and offering a foundation for further phylogenomic and conservation studies within Arecaceae.

De novo assembly and phylogenetic analysis of the complete chloroplast genome of Amorphophallus maxwellii Hett. 1994 (Araceae)

Abstract Amorphophallus, valued for glucomannan-rich corms and ornamental value, includes A. maxwellii Hett (1994), a tuberous geophyte from southwestern Thailand adapted to seasonally dry tropical biomes. We sequenced its complete chloroplast genome: 179,466 bp with a typical quadripartite structure (LSC 94,917 bp, SSC 15,775 bp, IRs 34,387 bp each), containing 128 genes (83 protein-coding, 37 tRNAs, and 8 rRNAs), and 34.13% GC content. Phylogenetic analysis of 15 complete chloroplast genomes strongly placed A. maxwellii within clade CA‑II as sister to A. kachinensis, supporting monophyly of lineages SEA, CA‑I, and CA‑II. This genomic resource facilitates evolutionary and stress-tolerance studies in Amorphophallus.