Comparative evaluation of germ cell-specific promoters identifies robust drivers of transgene expression during mouse spermatogenesis

Coupling a developmental promoter to CRISPR interference for Wnt pathway regulation in human pluripotent stem cells

While directed differentiation of human pluripotent stem cells commonly relies on the timed delivery of extracellular factors, these uniform treatments often yield heterogeneous responses across cell populations. Linking intracellular gene regulation directly to an emerging developmental state offers a complementary st...

Antisense Oligonucleotide-Mediated XIST Knockdown Modulates Endogenous Pluripotency-Associated Transcription Factor Expression in Porcine Female Somatic Cells

Simple Summary Somatic cell reprogramming is an important technology for animal biotechnology, embryo engineering, and genetic improvement. However, the efficiency of reprogramming is limited by epigenetic barriers that restrict the activation of pluripotency-related genes. The long non-coding RNA XIST plays a central...

Mechanisms and functional implications of long-range enhancer-dependent gene regulation

Generation of a Highly Efficient and Chemically Inducible Gene Expression System

Background: Chemically inducible gene expression systems provide precise control over the temporal and spatial expression of genes. They are powerful tools for analyzing gene function during plant development and can effectively avoid the issues associated with transgene expression driven by constitutive promoters. The...

Allele-specific expression is associated with lower translational efficiency in mouse embryos and stem cells

An mRNA’s cytoplasmic fate can be shaped by its transcriptional history, its modifications and associated protein complexes. Allele-specific expression (ASE), in which a gene’s two alleles are transcribed unequally, provides a natural experiment given that the alleles can differ in sequence or chromatin state, yet thei...

Evaluation of eef1a1l1 expression stability and promoter-driven reporter activity during zebrafish development

Housekeeping genes are widely used as experimental references for gene expression, yet many exhibit substantial variation within and between cell types. Using Zebrafish Meta Atlas Project (ZMAP), we found eef1a1l1 to be ubiquitously and highly expressed across zebrafish embryonic cell types, developmental stages, and s...