TL;DR
This chapter presents a live-cell, super-resolution microscopy approach based on Total Internal Reflection Fluorescence (TIRF), using planar IS as an in vitro model to investigate actin and tubulin dynamics in primary human CD4 T cells.
This work shows that SWAP70 is rapidly recruited to regions of intense actin remodeling at the synaptic interface, where it forms a stable network with slower turnover than filamentous actin, indicating that SWAP70 cooperates with the linear actin filament to help stabilize the cortical cytoskeleton.
Branched actin polymerization mediated by the actin-related protein 2/3 (Arp2/3) complex provides the primary pushing forces for a variety of cellular processes, including cell migration, endocytosis, and phagocytosis. Myosin-I motors, which frequently colocalize with branched actin networks at the cell leading edge, h...
In the process of mounting humoral immune responses, B cells typically engage antigens on the surface of antigen-presenting cells (APCs) via the immunological synapse (IS). To study this critical cell interaction structure, we developed a dynamic micropatterning technique that models IS formation with precise spatial a...
This study developed an integrated approach combining mass spectrometry, immunofluorescence, super-resolution imaging, subcellular structural analysis, and three-dimensional (3D) reconstruction that is well-suited for investigating Lacrymaria and broadly applicable for exploring the presence and biological functions of...
The actin cytoskeleton generates diverse cellular functions, including intracellular positioning, symmetry breaking, and cell migration, through dynamic self-organization. Despite extensive molecular characterization of actin-binding proteins, the physical principles by which local molecular interactions give rise to c...
Immunotherapies affect T cell function by modifying the immune synapse, which forms at the interface between T cells and their targets. Phosphoprotein associated with glycosphingolipid-rich microdomains 1 (PAG) is a T cell adaptor protein within the immune synapse that mediates signaling by the inhibitory co-receptor P...
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