Structure-guided discovery and engineering of miniature CRISPR-Cas12m for epigenome editing.

Rational and computation-assisted engineering of a compact and efficient CRISPR-Cas12f genome editor.

The CRISPR-Cas12f system is an ultracompact genome-editing platform, yet only a few orthologs exhibit robust activity in mammalian cells. Here, we systematically screened 23 Cas12f orthologs and identified two active nucleases, PspCas12f1 and TcCas12f1, capable of genome editing in human cells. sgRNA scaffold optimizat...

Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.

An enhanced CgCas12n system (eCgCas12n) is established that enables efficient mammalian genome editing and provides an efficient genome- and base-editing platform for functional genetic studies.

MODULAR CLICK-CHEMISTRY-BASED ENGINEERING MINIMALLY PERTURBS CRISPR RIBONUCLEOPROTEIN FORMATION FOR GENOME IMAGING APPLICATIONS

CRISPR/Cas Systems: Biological Basis and Genome Editing Applications.

Clustered regularly interspaced short palindromic repeats (CRISPR) and associated (Cas) systems have revolutionized the field of genome engineering by providing versatile, efficient, and programmable tools for precise genetic manipulation. Originally identified as an adaptive immune mechanism in prokaryotes, CRISPR/Cas...

Enhanced CRISPR-Cas3-mediated genome editing using circularized crRNAs

Summary Type I-E CRISPR-Cas3 represents a genome-editing technology in which large deletions averaging several kilobases are introduced in target regions. However, its genome-editing efficiency varies considerably across targets and cell types, making it difficult to achieve consistent results. Here, we investigated th...

Engineering TnpB as a compact RNA-guided genome editor from molecular constraints to design principles.

Recent discoveries of non-CRISPR RNA-guided nucleases have expanded the landscape of programmable genome editing beyond canonical Cas systems. Among these, TnpB proteins derived from IS200/IS605-family transposable elements are ultra-compact RNA-guided DNA endonucleases with potential for delivery-constrained genome en...