Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Beyond gene editing: the age of chromosome-scale genome engineering.

How advances in large DNA assembly, synthetic biology, and genome-wide validation are making chromosome-scale genome engineering an increasingly tractable experimental framework for understanding, building, and repairing genomes is discussed, while outlining the analytical requirements and current technical barriers th...

A multiplex genome editing pipeline for rapid combinatorial trait engineering.

A multiplex genome engineering pipeline capable of introducing up to eleven genomic modifications across seven different genes simultaneously in viable founder mice is established, advancing methods of rapid establishment of complex genetic models, with a wide array of applications.

High‐Content CRISPR Screening: Methods and Applications

This review synthesizes the methodological evolution of CRISPR screening, emphasizing advances in perturbation strategies, delivery systems, and multimodal readouts to demonstrate refined gene network annotations.

Precision genetic manipulation: CRISPR toolkits reshape plant biology.

Key CRISPR-derived tools are introduced, including base editors, prime editors, and programmable transcriptional and epigenetic regulators, and remaining challenges to stimulate future exploration of the full potential of CRISPR toolkits are outlined.

Programmable enzymes for targeted gene insertion.

EMERGING APPLICATIONS OF CRISPR–CAS9 BEYOND GENE EDITING

This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.