gene editing

LaMGen: LLM-based 3D molecular generation for multi-target drug design

This study introduces LaMGen, an LLM-powered framework that leverages large-scale protein-ligand data and rotation-aware molecular encoding to rapidly produce chemically plausible multi-target candidates, achieving strong zero-shot generalization, superior molecular quality, and robust performance across dual- and trip...

In Silico Design and Evaluation of a CRISPR dCas9 DNMT3A DNMT3L Epigenetic Editing System Targeting the CDKN2A Promoter

CDKN2A (p16) is a tumor suppressor gene whose normal function is to stop the cell cycle from over dividing, allowing cells to enter senescence after completing their function. When the CDKN2A promoter is methylated, the gene is silenced, and cells continue dividing rather than stopping, a mechanism shown in published r...

Safe and Stable Germline Transmission of MSTN Mutations in Cattle

ABSTRACT With the global population expected to reach 10 billion by 2050, sustainable livestock production is critical. Gene editing of the myostatin (MSTN) gene represents a promising strategy to enhance muscle growth in cattle. In this study, MSTN‐mutated founder (F0) cows were used to generate F1 offspring via ovum...

CRISPR/Cas9-mediated targeted mutagenesis of GmMYB100 to increase isoflavone content in soybean seeds

基于生物网络的动态拓扑优化

This paper explores the application of dynamic topology optimization techniques to biological networks, aiming to enhance drug discovery and gene editing. Biological networks, complex systems of interconnected nodes and edges, are increasingly utilized in various fields. Traditional optimization methods often rely on f...

A national framework for managing dual-use research of concern: integrating biosecurity, public health, and research governance

Dual-use research of concern (DURC)—legitimate life-science research that could be misapplied to cause significant harm—has become an increasingly urgent policy challenge. Advances in synthetic biology, gene editing, and artificial intelligence have expanded both the pace and diffusion of high-risk biological work, whi...

Molecular Scissors With a Search Bar: A Historical Development Review of CRISPR and the Editing of the Genome

This article presents a narrative review of CRISPR and the Editing of the Genome in the context of Biology. The literature on this topic has expanded substantially over recent decades, yet it remains fragmented across subfields, methods, and national research traditions. Drawing on an interpretive synthesis of represen...

Lipopolymer Nanoparticle‐Mediated In Vivo CRISPR‐Cas9 Editing of Mrg15 Restores Mitochondrial Mitophagy to Alleviate Metabolic‐Associated Steatohepatitis

Metabolic‐associated steatohepatitis (MASH) remains difficult to treat due to the lack of interventions capable of targeting upstream disease drivers and achieving durable disease modification. The epigenetic regulator Mrg15 has been implicated in mitochondrial dysfunction and metabolic stress in the liver, suggesting...

Insights into fodder quality enhancement in sorghum through genetic and molecular approaches

Forage sorghum (Sorghum bicolor L. Moench), a climate-resilient, drought-tolerant fodder crop with high adaptability and biomass potential, plays a significant role in addressing global livestock feed and fodder demands. However, its complex quantitative quality traits, such as crude protein, fibre fractions, crude fat...

Hereditary transthyretin amyloidosis with polyneuropathy (ATTRv-PN): integrating epidemiological insights with diagnostic and therapeutic progress

Hereditary transthyretin amyloidosis with polyneuropathy (ATTRv-PN) is a progressive, fatal multisystem disorder characterized by significant genotypic and geographic heterogeneity. Despite recent therapeutic breakthroughs, overlapping clinical features frequently lead to misdiagnosis and delayed intervention....

Targeting TaNSUN2 provides a potential route to combine antiviral resistance with improved productivity in wheat

Research on crop disease has shifted from a single pathogen-host framework toward a multifactorial host-microbiota-environment framework. This transition, captured by the pathobiome concept, redirects attention from individual pathogens to the broader biological networks that condition disease establishment, progressio...

Deciphering abiotic stress resilience in crop plants through multiomics insights and CRISPR Cas9 mediated genome editing

Abiotic factors, such as drought, salt, severe temperatures, and heavy metal toxicity, persistently threaten global agricultural production, contributing to an estimated 40–70% of yield losses in primary food crops globally. Drought diminishes yields by as much as 50% in rice, 42% in soybean, 40% in maize, 21% in wheat...