gene editing

Heterogeneous CRISPR/Cas9 Editing of HMOX1 Is Associated with Altered Heme–Biliverdin Metabolism and Basal Stress-Associated Transcriptional Programs in Chicken LMH Cells

Findings show that heterogeneous HMOX1 editing and reduced HO-1 abundance are associated with disruption of the avian heme–biliverdin metabolic axis and coordinated remodeling of basal immune, stress, extracellular signaling, and cell-cycle-associated transcriptional programs in Chicken hepatocellular carcinoma-derived...

Bridging Traditions: Integrating TCM Into ERAS for Enhanced Gastrointestinal Recovery —A Scoping Review

Evidence stratification showed standardized EA has moderate-certainty benefits for shortening flatus and defecation time and lowering postoperative ileus incidence, whereas herbal and external treatments only have low-certainty evidence restricted by small cohorts, inadequate blinding and inconsistent operating protoco...

Restoring the Balance: CRISPRa-Driven β-Tubulin Compensation as a Strategy for Tubulinopathy Treatment

By increasing the expression of various beta-tubulin proteins, this work demonstrates a restoration of the microtubule network and primary cilia formation and develops a mutation-independent treatment strategy based on the upregulation of non-mutated beta-tubulin isotype protein using CRISPR-Cas9 activation.

Targeted Genetic Knockout of Can f 1, the Major Allergen in Dogs.

It is demonstrated that targeted genetic knockout of the major dog allergen is compatible with canine development and can abolish the IgE-mediated allergic response, supporting the feasibility of a gene-based approach to reducing canine allergenicity.

Lipopotrein(a): from the complex metabolism to the optimal lowering drug.

In conclusion, Lp(a) is a key determinant of residual cardiovascular risk and a better understanding of its pathophysiology and the development of targeted therapies may improve cardiovascular risk stratification and prevention strategies.

BraALA3 Homologues Mediate Propiconazole Effects on Growth of Flowering Chinese Cabbage.

Three P4-ATPase flippase homologues aminophospholipid ATPase 3 in flowering Chinese cabbage that function as sensitive targets for PCZ are identified and provide a precise molecular target for developing PCZ-free germplasm in flowering Chinese cabbage through gene editing.

A transcription factor-focused CRISPR screen identifies SKI as a BCL11A-independent repressor of ζ-globin.

Findings establish SKI as a direct, BCL11A-independent transcriptional repressor of ζ-globin, which advances the understanding of globin gene regulation and suggests targeted ζ-globin reactivation as a potential therapeutic strategy for α-thalassemia.

Precision Engineering of Evolution-Resilient Rice against Bacterial Blight.

The persistent conflict between rice and Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight, exemplifies a dynamic genetic arms race in agriculture. The cyclical deployment and erosion of major resistance (R) genes highlight the high adaptive potential of Xoo and the need for strategies that are...

Morpholino Mediated Gene Editing: A Gene Knockdown Technique Using Translational Blocking Morpholino Oligonucleotides in Single-Celled Cavefish Eggs

PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System

Gene therapy for hereditary deafness: Progress, challenges and translational implications from OTOF to GJB2

Early inner‐ear gene‐therapy efforts, clinical and preclinical developments in OTOF targeted therapy, and current GJB2 directed strategies, including gene replacement, cell‐specific delivery, base editing, pharmacological rescue, and potential in utero intervention are summarized.

All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System