gene editing

Engineering Immune-Protective Stem Cell-Derived β-Cell Grafts for Type 1 Diabetes: Encapsulation Technologies and the Emerging Graft Ecosystem.

BACKGROUND Stem cell-derived β-cell replacement has emerged as a promising strategy for the treatment of type 1 diabetes (T1D), potentially addressing the limitations of exogenous insulin therapy and donor-dependent islet transplantation. However, despite substantial progress in stem cell differentiation and transplant...

Molecular Bases and Genetic Design of Rice Disease Resistance for Optimized Yield and Sustainable Agriculture

ABSTRACT Rice diseases continue to undermine yield stability and threaten the sustainability of rice production. The central challenge is therefore not simply to maximize immune activation, but to identify genetic interventions that remain effective across diverse pathogen races and environmental conditions without imp...

Correction: Editorial: CNS immunity – paradox of maintenance, repair and destruction

selective gateways for antigen exchange, immune cell trafficking, and bidirectional signaling. Pathways via the olfactory and vagus nerves, coupled with neuroendocrine axes, further integrate CNS immunity with systemic physiology. The gut-brain axis, mediated by microbiota-derived metabolites and immune signaling, exem...

Bibliometric mapping and trend analysis of RNA interference-related patient-derived organoid research in colorectal cancer

RNA interference-related perturbation and patient-derived organoid models have increasingly appeared as complementary experimental approaches in colorectal cancer research. However, this literature overlaps with CRISPR-based gene editing, noncoding RNA biology, extracellular vesicle studies, spheroid models, metabolic...

Integrative Techniques for Hybrid Breeding in Eucalyptus: Advances, Innovations, Challenges, and Future Prospects

The aim of this review is to present integrative techniques for hybrid breeding in Eucalyptus based on conventional methods such as phenotypic selection and controlled hybridization, and modern genomic-assisted approaches to guide product development.

Universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids

A series of universal donor vectors are generated to enable biallelic integration of different degron tags using different selection strategies (FACS, drug selection) and can be fused in-frame to endogenous protein C-termini via homology-independent repair following Cas9-induced double strand breaks.

Interrogation of noncoding schizophrenia risk variants using CRISPR-based functional genomics

Findings show distinct noncoding mechanisms that map within GWAS signals, and provide a path forward for interrogating noncoding regulatory elements and variants in disease loci.

Parthenolide boosts megakaryocyte maturation and restores platelet responses in Wiskott–Aldrich syndrome

Wiskott-Aldrich syndrome (WAS) is an inborn error of immunity with a broad disease spectrum, classified into class I (late onset) or II (early onset) variants. Thrombocytopenia and small platelets are the most consistent findings among patients, difficult to treat and related to development of autoimmunity. To identify...

Infectious transgenesis in arthropods

In the last decade, there has been an explosion in the development of in vivo germline genetic editing technologies in arthropods, first pioneered by the Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) method. While ReMOT Control and related technologies (DIPA-CRISPR and SYNCAS) have shown great utility f...

Nanomedicine‐driven innovations in postoperative cancer immunotherapy: Remodeling tumor microenvironment and precision delivery strategies

Postoperative recurrence remains a critical challenge in cancer management, necessitating innovative strategies to reshape the tumor microenvironment (TME) and enhance immune surveillance. This review highlights nanomedicine‐driven breakthroughs in postoperative immunotherapy, emphasizing their potential to overcome...

A heuristic graph editing approach to correlation clustering with overlap

Correlation clustering seeks a partition of the vertex set of a given graph/network into groups of closely related, or just close enough, vertices so that elements of different groups are not close to each other. The problem has been previously modeled and studied as a graph editing problem, namely Cluster Editing , wh...

Gene editing using large insertions.