gene editing

Graft Biology in the CRISPR Era: From Tissue Fusion to Genome Compatibility

ABSTRACT Plant lineage has traditionally constrained grafting compatibility, with monocots generally considered incompatible because of their dispersed vascular bundles and limited secondary growth. Recent studies have shown that embryonic grafting can establish successful graft unions in selected monocot systems by ex...

Gene therapy in Alzheimer’s disease (AD)

Gene therapy in Huntington’s disease (HD)

Gene therapy in Duchenne muscular dystrophy (DMD)

Multiomics Reveals IGF-1 c.258A > G Reduces Triacylglycerol Containing Medium-Chain Fatty Acid in Milk

Fatty acid composition of milk triacylglycerol (TAG) is important for neonatal growth. We previously identified a synonymous mutation widely distributed in human populations, IGF-1 c.258A > G, which decreased milk TAG content in gene-edited mice. So far, the acyl chain composition of affected TAGs, underlying molecular...

Microbiome–Tumor–Host Interactions in Cancer Therapy: From Chemoresistance Mechanisms to Engineered Living Medicines

This review synthesizes recent advances in pharmaco‐microbiomics, reframing cancer treatment in a hologenetic context, with host, tumor and microbiome acting as a tripartite entity. We comprehensively review microbiome‐mediated regulation of chemotherapeutic responses, encompassing both chemosensitization and resista...

CRISPR–Cas9 genome editing: technological advances, delivery strategies and precision engineering of primary cells for therapeutic application in genetic disorders and diabetes mellitus

Human-Specific Regulation of CHD2 Controls the Tempo of Cortical Development through Lysosomal Homeostasis

It is demonstrated that CHD2 dosage bidirectionally regulates lysosomal function and autophagosome flux to set the tempo of neuronal maturation, and that CHD2 and lysosomal homeostasis constitute a critical molecular axis regulating the pace of cortical development across evolution and disease.

Biotechnology in the Battle Against Potyviruses: A Review of Disease Management in Solanaceae

Abstract Some Solanaceae members are among the most important agricultural commodities cultivated globally. Climate change poses a threat of increased pathogen infections in solanaceous plants, with viruses (especially potyviruses) being among the most difficult to control. Over the past few decades, various methods ha...

INSCRIBE STAR Protocols: Code and demonstration data for multiplexed smFISH and ratiometric barcode readout

This repository contains the analysis code and demonstration data accompanying the INSCRIBE STAR Protocols manuscript. The workflow processes multiplexed smFISH imaging followed by ratiometric barcode readout in HEK293 cells, integrating gene-expression measurements and barcode editing levels at single-cell resolution....

CRISPR-Cas9 in Myelodysplastic Syndromes: The Potential of Editing DNMT3A, TET2, ASXL1, TP53, and SF3B1 to Prevent Disease Progression—A Literature Review

Myelodysplastic syndromes are clonal hematopoietic stem cell neoplasms characterized by ineffective hematopoiesis and a variable risk of transformation to acute myeloid leukemia. This study examines the potential of CRISPR-Cas9 editing of DNMT3A, TET2, ASXL1, TP53, and SF3B1 to prevent disease progression. A narrative...

INSCRIBE STAR Protocols: Code and demonstration data for multiplexed smFISH and ratiometric barcode readout

This repository contains the analysis code and demonstration data accompanying the INSCRIBE STAR Protocols manuscript. The workflow processes multiplexed smFISH imaging followed by ratiometric barcode readout in HEK293 cells, integrating gene-expression measurements and barcode editing levels at single-cell resolution....