gene editing

Navigating Quality, Safety, and Analytical Testing Frameworks for Viral Vector–Based Gene Editing Therapies

This article aims to provide a working framework for verifying the potency, genomic integrity, and clinical safety of vector-based gene therapies—one intended to be useful both to laboratories developing these products and to those responsible for regulating them.

Comparative chloroplast genomics of six Bupleurum (Apiaceae) accessions: candidate barcodes, phylogeny based on available plastomes, and candidate RNA-editing sites

The whole-plastome phylogeny recovered Bupleurum as monophyletic relative to Chamaesium, and only one of seven multi-accession operational binomial groups was monophyletic, and only one showed a positive local barcode gap.

Long-term in vivo CRISPR/Cas9 gene editing in liver triggers host immunity and clonal mutations in DNA damage response genes beyond off-target effects.

Integrating Multi-Omics in Aquaculture: From Genetic Gains to Sustainable Aquaculture

This review outlines foundational applications of omics in aquaculture and highlights the characteristics and current applications of distinct omics approaches, and summarizes three core application domains of omics in aquaculture.

Establishment of a CRISPR/Cas9-mediated system for targeted editing of the MFS gene in mint

A substantial decrease in menthofuran content in the essential oil of the edited line #10 compared to the wild-type control is revealed, thereby demonstrating a viable strategy for improving mint essential oil quality through genome-editing.

Mutation-induced phenotypic plasticity drives formation of a pro-metastatic tumour-intrinsic niche

It is argued that formation of a tumour-intrinsic niche is a prerequisite for BRAF-mutant CRC seeding to distant organs and that interference with niche formation may help avoid metastatic relapse.

An Explicit Interaction-Prompted Diffusion Framework for High-Fidelity 3D Molecular Generation.

Current structure-based drug design generative models often struggle to faithfully recapitulate genuine ligand-protein binding interactions. Instead, under the coupling of implicit learning architectures and biased training data, they tend to learn spurious statistical correlations. To address this, we propose EIP-Diff...

Universal CAR-T cell therapy: mechanisms and evasion strategies of bidirectional immune rejection.

This review systematically delineates the mechanisms underlying bidirectional immune rejection and comprehensively summarizes state-of-the-art mitigation strategies and highlights the development of alternative, inherently hypoimmunogenic cell sources as a fundamental approach to circumventing these immunological barri...

Advanced gene editing technologies for oncology mechanisms, applications, and clinical implementation

This review highlights the transformative impact of gene editing on cancer medicine, advancing toward more accurate, effective, and personalized therapeutic approaches.

Analysing long-read CRISPR experiments with CRISPRLungo.

This work presents CRISPRLungo, a computational pipeline specifically designed for long-read amplicon sequencing of gene edited samples that incorporates unique molecular identifier-based error correction and statistical filtering to distinguish true editing events from background noise, enabling robust detection of sm...

Identification of root system architecture associated genes, regulatory network, and expression analysis under abiotic stress in maize.

expression modules suggest that Zm00001eb403030 (RTCL1) and auxin-associated regulators modulate post-embryonic root initiation and branching, and the current investigation outlines a stress-responsive maize RSA network and identifies targets for functional validation, genome editing, and breeding climate-resilient cul...

Gene-edited hypoimmune islets as a cure for type 1 diabetes: a review of the immunological challenges.

Current preclinical and clinical evidence from 2019 to 2026 for gene-edited hypoimmune islets is critically evaluated, highlighting key immunological vulnerabilities that may emerge over time and whether these long-term challenges can be overcome.