Combinatorial CRISPR/Cas12a excises targeted loci in the mammalian genome at very low efficiency
Findings indicate that the Cas12a system does not yield locus excision at the efficiency required for sensitive negative selection assays, and showed no meaningful correlation between gRNA efficiency, orientation, GC content, or cut site geometry and the success of locus excision.
N. Anvar, Zsofia M. Szegletes, R. Steger et al.
· bioRxiv · 0 citations