Yuki Naka

Softening a Salt Bridge Improves the Low-Temperature Activity of a Mesophilic Enzyme While Largely Preserving Its Thermal Stability

Mesophilic enzymes are often less active at low and moderate temperatures than their psychrophilic homologues. The earthworm Eisenia fetida has cold-adapted enzymes, but the end-β-1,4-mannanase shows weaker activity at low temperatures compared with other E. fetida enzymes. Cold-adapted enzymes need flexible structur...