Targeted Mutations in d-ldh and budA Differentially Affect Adaptive Traits and Fermented Milk Quality in Companilactobacillus crustorum MN047
This study examined how targeted mutations in two pyruvate-associated genes, d-ldh and budA, affected adaptive phenotypes and fermented milk quality in Companilactobacillus crustorum MN047. Premature stop codons were introduced into d-ldh and budA by CRISPR-assisted base editing and verified by Sanger sequencing. The r...
Jia Li, Pan-Pan Wang, Zi-Wen Qu et al.
· Fermentation · 0 citations