NDAORep accelerates the evolution of regulatory elements, protein functions, and metabolic pathways, establishing a powerful platform for metabolic engineering and synthetic biology.
Fusarium venenatum is the most widely used filamentous fungus for biomass-based microbial protein production mainly used as meat analogs. However, insufficient iterative genome editing and lack of synthetic biology tools for fine-tuning gene expression limited engineering of F. venenatum for improved traits. Here, repl...
S. Gu, Na Wang, Ya-Dong Tu et al.· Metabolic Engineering· 0 citations
A multilevel engineering strategy to enhance yeast protein production by optimizing precursor supply, translation machinery, and diploid construction provides both improved protein content and key targets for breeding high-protein microbial strains.
Yang Liu, Cai-Ying Jin, Wentao Shen et al.· Bioresource Technology· 1 citation
Base editors (BEs) enable efficient A-to-G or C-to-T conversions without double-stranded DNA cleavage, but their editing windows remain difficult to tune, limiting genome engineering flexibility. Here, we engineered CRISPR/Cas12b sgRNA by introducing MS2 hairpins to recruit an MS2-N55K-cytidine deaminase-UGI complex, e...