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Author

Yaokang Wu

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#gene editing Oct 2026

Replicon engineering-driven modular synthetic biology toolkit for efficiently iterative genome editing in Fusarium venenatum.

Fusarium venenatum is the most widely used filamentous fungus for biomass-based microbial protein production mainly used as meat analogs. However, insufficient iterative genome editing and lack of synthetic biology tools for fine-tuning gene expression limited engineering of F. venenatum for improved traits. Here, repl...

S. Gu, Na Wang, Ya-Dong Tu et al. · 0 citations
Jul 2026

Precursor supply and translational machinery engineering of Saccharomyces cerevisiae for improving cellular protein content and biomass-based microbial protein bioproduction.

A multilevel engineering strategy to enhance yeast protein production by optimizing precursor supply, translation machinery, and diploid construction provides both improved protein content and key targets for breeding high-protein microbial strains.

Yang Liu, Cai-Ying Jin, Wentao Shen et al. · 1 citation
#gene editing Aug 2026

Design of base editing systems with tunable editing hotspots within a defined window through sgRNA engineering.

Base editors (BEs) enable efficient A-to-G or C-to-T conversions without double-stranded DNA cleavage, but their editing windows remain difficult to tune, limiting genome engineering flexibility. Here, we engineered CRISPR/Cas12b sgRNA by introducing MS2 hairpins to recruit an MS2-N55K-cytidine deaminase-UGI complex, e...

Wen-Liang Hao, Lai-Chuang Han, Yao-Kang Wu et al. · 0 citations

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